摘要:

A study was made to investigate the biological principle of new attachment of connective tissue to periodontally diseased root surfaces utilizing the previous finding that topical application of citric acid to the affected area may stimulate periodontal regeneration. Reconstructive surgery was performed on ten surfaces of nine teeth involved in advanced chronic periodontal disease. A muco‐periosteal flap was raised and the apical extent of existing subgingival calculus was demarcated by a notch made through the calculus and into the root. Following thorough instrumentation, a saturated solution of citric acid was applied to the root surface for five minutes and the flap repositioned and sutured. Four months later the teeth with attached periodontal tissues were removed and processed for histological analysis. Connective tissue regeneration characterized by deposition of new cementum, and more coronally, by tightly apposed soft connective tissue had occurred in all specimens. The junctional epithelium ended 1.2–2.6 mm coronal to the apical border of the notch in the various specimens. The results demonstrate that regeneration of periodontal tissues to a root surface that has become denuded as a result of chronic, destructive periodontal disease and that has been covered by calculus is, in fact, a biological possibility. This study does not establish whether ornotacid conditioning of the root surface is a prerequisite for new attachm

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1980.tb00256.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Eighteen periodontally diseased teeth and eleven normal teeth requiring surgical removal were each treated with one of four different solutions to elute potentially toxic material from their root surfaces. The solutions used were pyrogen‐free water, phenol‐water, trichloroacetic acid and citric acid. To define the composition of the eluted material, samples were analyzed for calcium, for ketodeoxyoctonic acid (KDO), a carbohydrate unique to Gram‐negative bacteria, and for limulus lysate activity. Limulus‐positive material was then analyzed for protein and nucleic acids and subjected to heat treatment, to the enzymes RNAse A and T1, and to lysozyme.It was found that TCA and citric acid removed more calcium and more toxic material from the root surface and subsurface than did either water or phenol‐water.The material extracted from 13 of the 18 diseased roots was limulus‐positive. Four of the limulus‐positive samples were also KDO‐positive, strongly indicating the presence of endotoxin in these samples. Neither these nor any of the other limulus‐positive responses were reversed or significantly reduced by exposure of samples to heat, to RNAse A and T1, or to lysozyme. In addition, limulus‐positive responses were unrelated to the amounts of protein and nucleic acids in the extracted material.When considered together, these preliminary characterizations indicate the presence of endotoxin in the material eluted from periodont

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1980.tb00257.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Connective tissue cells responding to wounding of the periodontal ligament of the lower first molar in mice were studied using the techniques of radioautography and grain counting. Animals were given an intraperitoneal injection of 2 uCi 1g3H‐Tdr 1 hr before being killed at either 30, 72 or 120 hr after wounding. The ligament in 1 um plastic sections was divided into compartments on the basis of distance from the wound, and the relative number of labelled cells in each compartment was assessed at 30, 72 and 120 hours after wounding. The distance of each labelled cell from the closest blood vessel was also measured at each time to detect relative movement of labelled cells away from blood vessels. In a Parallel experiment, haematogenous progenitors of macrophages were depleted by irradiating the animals with 800rads prior to woundilng to determine if mistakes in identification between fibroblasts and marophages could significantly affect the results. The ultrastructural characteristics of 150 of the3H‐Tdr labelled cells was examined in thin sections of wounded periodontal ligament prepared for elctron microcope radioautography, The majority of cells lebelled 30 hours after wounding were confirmed to be paravascular, and most of them were found to be located within 200 μ of the wound margin. Some of these cells appreared to have divided a number of times between 30 and 72 hours after wounding, and to have migrated into the wound between 70 and 120 hours after wounding. Examination of the irradiated material and the electron microscope radio‐autographs suggested that significant numbers of macrophages had not been included in the counts of labelled cells. The elctron microscope radioautographs also suggested that cells which exhibited different degrees of cytodifferentiation had incorporated

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1980.tb00258.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

The present study monitored the healing responses at sites of attached but severed gingival fibers by following matrix production and turnover using3H‐proline in mice of different ages. Fifty‐six BNL short‐lived mice of mixed sex to 78‐week‐old were used. A vertical gingival incision was made at the mesial papilla of the right maxillary first molar to the alveolar bone crest severing the gingival fibers. The left side was used as a control. Periodontal regions adjacent to the incision and some distance from the incision were studied. All mice were given a s.q. dose of 2μCi of3H‐proline/gm. b. wt. and killed from 1 to 90 days later. Autoradiographs were prepared from routinely processed histologic sections for grain counting. The control crestal region exhibited the greatest matrical turnover of all sites tested regardless of age. With increasing age, however, the rates of uptake and turnover were significantly reduced in all regions. Post‐injury, additional peaks of activity occurred in young mice. In old mice, the injured cemental side exhibited significant uptake and prolonged matrical turnover. It was concluded that the matrix on the injured corium side was the most stable, while the cemental region was the least stable. Aging did not impede the tissue response to injury but increased the long

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1980.tb00259.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Eight human gingival fibroblast cell lines were cultivated on bovine collagen fibrillar mats thinly coated on over slips of tissue culture chambers. The responses of the cells to the collagen were studied by light and electron microscopy. It was shown that the collagenolysis was regional, occurring only in the immediate areas of the cells and resulted from the phagocytosis of collagen fibrils by the fibroblasts. There was no evidence by electron microscopy of an enzymatic lysis of the fibrils in the extracellular spaces. The fibrils were first seen within folds of the fibroblasts whose membranes contained multiple profiles of invaginating pinocytotic and/or membrane‐coated vesicles. The fibrils were then interiorized in slender cell processes and ultimately became enclosed within developing acid phsphatase positive lysosome‐like bodies. The process of phagocytosis and the sequences of development of the lysosomal enclosures are combined with the concept of “hydrolase secretion and recapture‐mediated endocytosis by fibroblasts” to derive a hypothesis for fibroblastic degradatin o

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1980.tb00260.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Regeneration of periodontal connective tissue attachment was studied at the ultrastructural level in cats following the removal of cementum and superficial dentin through a surgically created fenestration in the facial alveolar plate. Non‐decalcified specimens were examined 33, 45, 70, and 105 days after surgery. In early stages of healing, fibroblasts were aligned parallel, and immediately adjacent, to the cut, irregular dentin surface; subsequently, collagenous matrix formation was observed at this site. Some regions of the defects showed surface demineralization and extension of collagen into dentinal tubules. The first indication of cementum mineralization was the deposition of small crystallites along the irregularities of the dentin surface. After further development, a well‐defined zone containing c1osel packed crystallites was usually evident at the new dentin‐cementum junction. Peripheral to this, collagen orientation in the calcified cementum was primarily parallel to the tooth surface, with some perpendicular orientation suggestive of Sharpey's fiber formation. Collagen‐mineral patterns typical of the normal cementum surface were observed, and cementoblasts were seen in various stages of inclusion into the calcified matrix. Where the injury had not reached the dentin, collagen fibrils of new and old cementum were interwoven, and the junctional region demonstrated incomplete mineral

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1980.tb00261.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

The amount and concentration of protein present in fluid obtained from individual healthy and inflamed gingival crevices have been examined. It was found that an artefactual hyperbolic relationship existed between protein concentration and fluid volume in fluid originating from healthy crevices, resulting in over‐estimation of protein content. In fluid originating from inflamed crevices, no statistically significant correlation existed between protein concentration and fluid volume, which is to be expected for an inflammatory exudate. The fluid dynamics of the crevicular region is therefore probably similar to that of the rest of the bod

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1980.tb00262.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Samples of gingival fluid and plasma were taken from ten patients with varying severity of periodontal disease. Group I comprised seven patients with abundant plaque. Their mean pocket depth at the sampling sites was 5.6 ± 1.27 mm. Group II consisted of three patients with minimal plaque and a mean pocket depth of 1.8 ± 0.24 mm. A comparative analysis by crossed immunoelectrophoresis was carried out. Qualitatively evaluated orosomucoid and the complement factor C3 were degraded in all the gingival fluid samples, in contrast to those of plasma. A quantitative assay of 15 separate proteins in the two biological fluids showed reduced concentrations of alfa1‐antichymotrypsin, alfa2‐macroglobulin and haptoglobin in most gingival fluids. Orosomucoid, alfa1‐antitrypsin and C3 levels were decreased in all the gingival fluid samples. Reference ranges should be stated separately for the two biological fluids. The correlation coefficients between the plasma and the gingival fluid concentrations of each protein were calculated. The co‐variation was significant for 13 of the proteins studied. The ratio of the individual protein concentrations in the gingival fluid to that in the plasma was then calculated for each patient. A twoway analysis of variance of the variables patient groups and protein ratios showed that both effects were significant. Gingival fluid is an inflammatory exudate of serumal origin, but its protein profile is clearly modified by the loc

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1980.tb00263.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Beagle dogs were treated with 0.1 % aqueous solution of benzethonium chloride or with water for 27 weeks and for an additional three weeks to ascertain whether or not the plaque inhibiting effect of BTC was altered because of initial long‐term treatment. The results of this study show 0.1 % BTC significantly reduced plaque and gingivitis in beagles for 27 weeks, This plaque inhibitory effect of BTC was maintained in the subsequent three weeks, suggesting that its initial 27 weeks usage did not result in a change of “oral flora” that affected its eff

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1980.tb00264.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY