ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb01552.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb01553.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The cutaneus T‐cell lymphomas (CTCL) arc a group of diseases characterized by malignant proliferations of CD4 positive T‐cells having monoclonally rearranged T‐cell receptor (TCR) genes. A recent study using monoclonal antibodies to two TCR β‐chain variable (V) region gene products showed preferential expression of the Vβ8 gene product in these tumors. The finding of predominant usage of a single Vβ gene would imply that selection by antigen is important in the etiology of these tumors. We have studied eight cases of cutaneous T‐cell lymphoma and one cell line derived from a patient with mycosis fungoides/Sezary syndrome, using an extended panel of antibodies to V region gene products. Contrary to the previous report, in our study expression of the Vβ8 gene product by tumor cells was not observed in any of the cases of CTCL or in the tumor cell line studied; preferential use of any of the variable region genes recognized by the antibodies in the panel was

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb01554.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Human epidermal melanocytes were examined for proliferation nuclei' various conditions in the presence or absence of all‐trans retinoic acid (RA). Under conditions which supported proliferation, RA at concentrations of 0.25–1.0 μg/ml inhibited cell growth bill was not cytotoxic. When melanocytes were cultured under conditions which by themselves did 1101 support growth, RA did not overcome the growth limitation. Treatment of melanocytes with RA altered their morphological appearance. Alterations included retraction of dendritic processes, increased flattening, and a slight darkening of the cytoplasm in some of the cells. However, when examined biochemically, there was no significant change in the amount of malanin per cell or in lyrosinase activity. RA also inhibited proliferation of six different malignant melanoma lines. Inhibition was observed over the same RA concentrations and over the same time course in the melanoma cells as was seen in melanocytes. Inhibition of melanocytic and melanoma cell proliferation was slowly reversed following removal of RA from the culture medium. These results indicate that RA can inhibit proliferation of melanocytic c

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb01555.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Thirty cases of poorly differentiated carcinomas of the skin were examined for the expression of vimentin. All cases expressed cytokeratins; in addition, 12 cases were positive for vimentin. These were all non‐reactive with antibodies to S100 protein, HMB45 and desmin. The finding of vimentin in poorly differentiated squamous cell carcinomas underscores the need for caution in the use of immunohistochemical stains for tumor typing. Cutaneous squamous cell carcinomas are an addition to the list of epithelial tumors which are known to coexpress vimentin intermediate filaments. Other carcinomas which consistently express vimentin include those of renal, endometrial, thyroid, pulmonary, ovarian, salivary gland, adrenal and more recently, those of breast and prostatic orgi

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb01556.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

We reviewed the histologic and direct immunofluorescence findings in skin biopsy specimens from 24 patients with cutaneous porphyrias. The histopathologic changes were characterized by cell‐poor subepidermal bullae, festooning of dermal papillae, thickening and hyalinization of dermal blood vessel walls, dermal sclerosis, and a mild perivascular infiltration of mono‐nuclear cells in the upper dermis. The characteristic pattern on direct immunofluorescence testing was fluorescence of thickened doughnut‐like blood vessels in the upper dermis, reflecting deposition of immunoglobulins and C3, and deposition of immunoglobulins or C3 or both at the dermal‐epidermal junction in most cases. These findings are characteristic but not specific for the porphyrias; they may also be observed in the pseudoporphyria syndromes and in other clinical settings. However, the immunopathologic findings are distinctive enough to be useful in differentiating the porphyrias and pseudoporphyria syndromes from other subepidermal bullous diseases such as epidermolysis bullosa acquisita and bullous pemphigoid. The most important diagnostic test in the evaluation of the cutaneous porphyrias still is measurement of porphyrins in urine, stool, an

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb01557.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Rheumatoid neutrophilic dermatitis is a rare entity reported to occur in association with severe rheumatoid arthritis. Three patients with seropositive rheumatoid arthritis and rheumatoid neutrophilic dermatitis are described. Clinically, the eruption is characterized by symmetric erythematous papules, plaques, and rarely vesicles on extensor skin surfaces. Light microscopy reveals a dermal neutrophilic infiltrate without vasculitis. Spongiotic intraepidermal blisters, subepidermal bullae, or papillary neutrophilic microabscesses may be seen. The histologic differential diagnosis includes the oilier dermatoses in which neutrophils predominate. Rheumatoid neutrophilic dermatitis is one of several neutrophilic dermatoses seen in association with rheumatoid arthritis. Suggesting that it may be part of a spectrum of neutrophilic cutaneous reaction patterns in a predisposed host.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb01558.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The majority of tinea capitis in southern Taiwan occur in adult women and are caused byTrichophyton violaceum.We report the histopathological Endings of a series of 10 cases of tinea capitis caused byT. violaceum, the largest such study to date. Our study provides new information regarding the process of hair infection, mechanism of black dot formation, and chronicity of infection caused by this fungus. The cuticle remains intact. The fungi enter the proximal cortex where the cuticle is immature. They then colonize the proximal keratinized cortex and generate septate hyphae which transform gradually into arthrospores as they arc carried upwards by the growing hair. At the infundibular level, the hair cortex is almost completely replaced by spores and swells, impeding further exit of the growing hair and causing the already weakened hair to coil up inside the infundibulum, forming a black dol. In one patient who had infection for more than 20 years, there were changes suggestive of cyclic rein‐lection of the same follicles which might contribute to the chronicity of die infectio

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb01559.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Clinical and histological differentiation between Jessner's lymphocytic infiltration of the skin (JLI) and lupus erythematosus (LE) may he difficult. Previous immunohistochemical studies using monoclonal antibodies on frozen sections have shown that the majority of inflammatory cells in JL1 and LE are T lymphocytes, whereas B lymphocytes are few or absent. We have performed an immunohistochemical study on formalin‐fixed, paraffin‐embedded tissue sections from seven patients with JLI and live with LE using monoclonal antibodies MT1 (pan T‐cells), OPD4 (helper/inducer T‐cells CD4), MT2 (mantle/one B and some T‐cells), MB2 (pan B‐cells), L26 (pan B‐cells), and LN 1 (germinal centre B‐cells). In both diseases, the‐majority of the inflammatory cells were T lymphocytes (MT1 positive), confirming the results others have obtained on frozen material. OPD4 positive cells were detected in varying numbers in all cases. However, the percentage of B lymphocytes tended to be higher in JLI than LE. LN1 was the most useful H‐cell marker in distinguishing JLI from LE. However, a combination of M T2 and LN 1 gave the most significant difference. We conclude that immunohistochemical analysis using a panel of monoclonal antibodies to T and B lymphocytes may be useful in differentiating JLI from LE, although there is still c

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb01560.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Epidermal growth factor (EGF) and transforming growth factor alpha (TGFα) are important keratinocyte mitogens. Their effects are mediated by a cell membrane receptor (EGFR), quantitative and qualitative abnormalities of which may be responsible for deranged keratinocyte proliferation and differentiation. We have therefore examined EGFR expression immunohistochemically in a variety of benign and malignant epithelial neoplasms using monoclonal antibodies to the extracellular and intracellular receptor domains. In benign tumours (virus wart, seborrhoeic keratosis, keratoacanthoma), there was an ordered pattern of EGFR expression. In malignant tumours (basal and squamous cell carcinoma), there was loss of membrane labelling and cytoplasmic accumulation of the receptor. In premalignant proliferations, there was loss of membrane receptor with either absent cytoplasmic EGFR (actinic keratosis) or cytoplasmic receptor accumulation (Bowen's disease). Evidence of truncated receptors was not found. We suggest that dysregulation of the EGFR may be important in the development of cutaneous epithelial malignancies but that grossly abnormal forms of the receptor do not occur

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb01561.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY