ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00731.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The B‐cell leukemia/lymphoma gene (bcl‐2) produces a unique protein product (BCLP) that is believed to protect lymphoid cells from apoptosis. The bcl‐2 gene is frequently rearranged in nodal follicular lymphomas as well as in diffuse lymphoproliferations, but has generally been regarded as most useful in the recognition of the former of these lesions. However, little is known regarding BCLP expression in cutaneous lymphoid infiltrates. Using an immunohistochemical technique and a monoclonal antibody (clone no. 124) the authors examined 67 examples of cutaneous lymphoid infiltrates – 31 cases of malignant lymphoma of the skin (MLS) and 36 examples of cutaneous lymphoid hyperplasias (CLH) – to determine if patterns of BCLP reactivity could distinguish CLH from MLS or primary from secondary involvement of the skin by malignant lymphoma. Fifty‐eight per cent of MLS cases were BCL‐positive, as were 33% of CLH. Three of four cases of follicular cutaneous lymphoma showed BCLP‐positivity in neoplastic follicles, whereas similar structures in cases of CLH with a follicular pattern were BCLP‐negative. Sixty per cent of primary MLSs and 57% of secondary lymphomas were reactive for BCLP. These data suggest that immunostains for BCLP are of little help in the separation of benign from malignant cutaneous lymphoid infiltrates, and that they are likewise incapable of separating primary from secondary MLS. BCLP immunostains may have a limited adjuvant diagnostic role in distinguishing reactive from neoplastic follicular lymphoid le

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00732.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The bcl‐2 protein prolongs cell life by inhibiting apoptosis. Its expression has been studied in a variety of normal tissues and lymphomas but there is minimal information available concerning bcl‐2 expression by benign and malignant cutaneous T‐cells. Therefore, we investigated bcl‐2 expression in a wide variety of cutaneous T‐cell infiltrates using one‐ and two‐color immunohistologic techniques, bcl‐2 was expressed by the majority of lesional CD3+T‐cells in most cases. This included 22Departments of 26 cases of mycosis fungoides (MF), 3/3 cases of non‐MF cutaneous T‐cell lymphoma, 5/5 cases of lymphomatoid papulosis, 4/4 cases of T‐cell rich cutaneous lymphoid hyperplasia, 2/3 cases of bullous pemphigoid, 2/2 cases of discoid lupus erythematosus and 1/1 case of lichen planus. Titration experiments and comparative studies of tonsil section positive controls revealed that, relative to mantle zone B‐cells, there was over‐ expression of bcl‐2 by a variable subset of T‐cells in most cases. Assessment of multiple biopsies in a subset of MF cases showed stable expression of bcl‐2 over intervals of up to two years. In contrast to the widespread expression of bcl‐2 in both early and advanced MF skin lesions, abundant expression of the nuclear proliferation antigen, Ki‐67, was skewed toward advanced MF skin lesions. Ten percnt or more Ki‐67+cells were present in 5% of patients with patches/thin plaques, 38% with moderate plaques, 64% with thick plaques and 100% with tumor nodules. Two‐color immunohistologic analysis combined with molecular biologic analysis of clonality in the cases of T‐cell rich cutaneous lymphoid hyperplasia indicated that bcl‐2 expression was both a polyclonal and multi‐lineage phenomenon, suggesting that it occurred by a physiologic rather than mutational mechanism. We conclude that bcl‐2 expression is a common feature of cutaneous T‐cell infiltrates that has minimal differential diagnostic value for distinguishing lymphomas from reactive T‐cell infiltrates. In early MF lesions, abundant expression of bcl‐2 and sparse expression of Ki‐67 suggested that the accumulation of tumor cells during the initial progression of MF may be facilitated by prolonged clonal su

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00733.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Lichen striatus (LS) is an uncommon dermatosis that generally affects children. The histopathology of LS often shows a polymorphic epidermal reaction process of variable lichenoid and spongiotic changes having no specific histopathological criteria or simulating other diseases. In the present study, we have evaluated the histopathological features of 41 biopsy specimens and the immunohistochemical aspects of 10 cases of LS. In 50% of the cases, we found histopathological and immunopathological features constantly present and allowing a correct diagnosis. In 9 cases, the histopathology was not specific, and in 10 cases, a clear differentiation from other diseases was not possible. Immunohistochemical study demonstrated a CD3+T‐lymphocytic infiltration in which CD8+cells surrounded necrotic keratinocytes and intraepidermal vesicles filled with Langerhans cells. These findings seem to corroborate the hypothesis that a somatic mutation of a keratinocytic clone could induce an autoimmune response of the hos

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00734.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Degeneration of the elastica and collagen fibres in skin biopsies from patients with acrodermatitis chronica atrophicans was studied with light and electron microscopy. Elastic fibres were involved in the infiltrative stage while the elastin plexus was still present. In the atrophic phase, only fragments of elastic and oxytalan fibres were seen and the elaunin plexus was absent. Some collagen fibres were surrounded by osmiophilic material. In all biopsies, myelin sheaths were collapsed without axon structures. Spirochetes could be demonstrated in 69% of the biopsies and were most numerous in infiltrative and nodular lesions. The loss of elasticity of the skin in the atrophic phase may be caused by the destruction of both elastic and elastin fibres.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00735.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

A 19‐year‐old woman with a cutaneous ciliated cyst on her buttock is reported. Histological, histochemical, and electron microscopic studies revealed ciliated cells, mucinous cells showing merocrine secretion, and areas of squamous metaplasia in the cyst wall. This is the first case of cutaneous ciliated cyst that contained non‐ciliated mucinous cells as a comp

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00736.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The histopathology of the skin in the ichthyosiform erythroderma associated with classical Conradi‐Hunermann syndrome has been rarely described. Skin biopsies from 2 infants with the classical features of Conradi‐Hunermann syndrome including ichthyosiform erythroderma contained hyperkeratosis, dilated ostia of pilosebaccous structures, and calcium salts in the stratum corneum detectable with special stains. An unusual feature of one case was dilatation of acrosyringeal structures. We hypothesize that dilatation of acrosyringeal structures is analogous to the plugging noted in pilosebaceous units. Taken together, the ichthyosiform erythroderma of Conradi‐Hunermann has a distinctive histologic appea

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00737.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00738.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00739.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY