摘要:

Carcinoembryonic antigen (CEA) is a well‐established marker for sweat gland differentiation in adnexal neoplasms. In contrast to previous assumptions, CEA does not represent a single oncofetal antigen but comprises a family of homologous glycoproteins, i.e. the classical CEA‐180, biliary glycoprotein (BGP), and non‐specific crossreacting antigens (NCA). The aim of the study was to evaluate the distribution of the respective glycoproteins of the CEA family in sweat gland neoplasms, as compared to normal sweat glands. A panel of mono‐specific antibodies was applied to a total of 83 samples of hyperplastic and cystic alterations of sweat glands, sweat gland neoplasms, and cutaneous metastases of different origin. Within a single group of neoplasms the immunohistochemical profile was rather consistent. Staining for both CEA‐180 and NCA‐90 indicated ductal differentiation of both eccrine and apocrine glands. Co‐expression of CEA‐180, NCA‐90, and BGP was consistent with differentiation towards the secretory part of eccrine glands or the transitional portion of proximal ducts. Neoplasms with signs of apocrine secretion showed a preferential immunoreactivity for NCA‐90 and BGP. In conclusion, a specification of the members of the CEA family may be of some value in the differential diagnosis of adnexal neoplasms, but not in the discrimination of sweat gland carcinoma from m

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb00770.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

In patients with alcoholic liver disease, IgA deposits are often found in the liver, kidneys and skin. The present study was undertaken to determine the specificity, sensitivity and characteristics of IgA deposition in the skin of a group of alcoholic patients with or without overt liver disease, and compare these with findings in non‐alcoholic cirrhotics. Twenty‐six out of 28 patients with alcoholic cirrhosis had IgA deposition in the skin. In contrast, only 6 out of 11 patients who were alcoholic without any clinical liver disease and 3 out of 13 patients with non‐alcoholic cirrhosis of the liver had IgA deposition. In the control group, only 1 out of 52 patients with various dermatoses (excluding IgA dermatoses) had this IgA deposition. Unlike other IgA dermatoses, such as Henoch‐Schoenlein pnrpura or dermatitis herpetiformis, IgA deposition in alcoholic liver disease is characterized by its presence in the basement membrane of the eccrine secretory coils. This particular pattern of IgA deposition can be helpful in the diagnosis of alcoholic liver disease or alcoholism since the specificity is 100% with a sensitivity

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb00771.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Growth of normal melanocytes, nevus cells and primary melanoma cells is enhanced by insulin/insulin‐like growth factor‐I (IGF‐I)in vitro.It has been shown that a melanoma cell line possesses the IGF‐I receptor which plays a role in activation of the chemotactic response. Little is known about thein vivoexpression of the IGF‐I receptor and its role in melanocytic lesions. In an immunohistochemical study, we investigated the expression of IGF‐I receptor in frozen sections of congenital pigmented nevi from 10 patients (ages 8 months to 4 yrs) using the monoclonal antibody αIR3, which specifically recognizes the extracellular alpha subunit of the IGF‐I receptor. The proliferative activity of the nevus cells was examined by staining with Ki67 monoclonal antibody (reactive with all actively cycling cells). IGF‐I receptor was found to be widely expressed by the cell surface of the nevus cells. Membrane staining was occasionally stronger in the superficial portion of the congenital pigmented nevi. In contrast, Ki67‐positive cells were only sparsely scattered throughout the nevi with some tendency to localization to the superficial portion.This study indicates thatin vivothe IGF‐I receptor is widely expressed by congenital pigmented nevus cells. As opposed to keratinocytes, in which IGF‐I receptor expression defines the proliferation pool of the normal and disordered epidermis, the IGF‐I receptor is expressed by all nevus cells, irrespective of their proliferative status.Further studies are needed to assess whether the IGF‐I receptor expression can serve as a marker for increased risk for development of malignancy in various types of

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb00772.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Normal p53 protein protects the genome after DNA damage by delaying replication and allowing DNA repair, mdm‐2 is a recently discovered protein that controls p53 activity by binding to and inactivating p53 protein. A negative feedback loop has been suggested in which p53 induces mdm‐2 expression. We have shown that doses of ultraviolet B radiation that cause mild sunburn clinically, produce thymine dimers in keratinocytes detectable by immunocytochemistry. This causes an elevation of p53 protein without a concomitant p53‐mediated induction of

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb00773.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

We report a series of five cases of dermatofibrosarcoma protuberans, four of which were fibrosarcomatous and all of which showed histologic and immunohistochemical evidence of focal myoid/myofibroblastic differentiation (accounting for up to 5% of each tumor). These lesions were identified amongst 208 cases of dermatofibrosarcoma protuberans including 24 examples of the fibrosarcomatous variant. Three of the five patients were male and two were females; all were adults (37–72 years). One case arose on the scalp and two cases each on the abdominal wall and upper trunk. All tumors were less than 5cm in diameter and preoperative duration ranged from 2 months to 10 years. In three cases with follow‐up there was no recurrence. Histologically, all tumors were typical fibrosarcomatous or ordinary dermatofibrosarcoma protuberans but for the presence of scattered to confluent nodules and bundles of eosinophilic spindle cells associated with well‐defined cytoplasmic margins and vesicular nuclei associated with focal stromal hyalinization. While the typical dermatofibrosarcoma protuberans areas were CD34 positive, the myoid areas were negative for this antibody and positive for smooth muscle actin and pan‐muscle actin. All tumors were desmin negative. Recognition of myofibroblastic differentiation in fibrosarcomatous dermatofibrosarcoma protuberans is important not only because it gives support to the theory of a fibroblastic/myofibroblastic line of differentiation for this type of tumor, but also because it might be a source of confusion with other myofibroblastic lesions (e.g. myofibromatosis, adult myofibroma), especially when small biopsies are ev

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb00774.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

The capacity of the skin to be stretched and to return to its resting position is correlated to the quantity and to the quality of the elastic fiber network. Although elastic fibers have been demonstrated in scars, the time course of their appearance in scars and their role in scar elasticity has not been elucidated. A study was therefore undertaken to evaluate the elastic fiber network in scars.The scars studied were from re‐excision specimens following a biopsy performed for a benign or a malignant process. A total of 182 scars were evaluated in patients of different age groups. Miller's elastic tissue stain, considered to be superior to Verhoeff's van Giesen stain, was used. No elastic fibers were detected in any of 116 scars which were of less than 3 months' duration. In 66 scars present for over 3 months, a progressive increase in elastic fibers was present, first as focal and thin fibers, then as diffuse and thicker fibers. For scars of the same duration, a regional difference was noted in that scars from the back contained more and thicker elastic fibers than those from the cheek. When patients were stratified according to age, no appreciable difference was noted in the density of elastic fibers in both new and old scars between the different age groups. These results show that the synthesis of elastic tissue fibers in scars is a function of duration and site of the sca

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb00775.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb00776.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb00777.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY