ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1990.tb01669.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

In the present study a comparative immunohistochemical study was performed on skin biopsies from of patients with Jessner's lymphocytic infiltration of the skin (LIS), polymorphous light eruption (PLE), discoid lupus erythematosus (DLE) and sub‐acute cutaneous lupus erythematosus (SCLE) using a large panel of monoclonal antibodies against T cell differentiation antigens (CD3, CD4, CDS), immunoregulatory T cell subsets (CD7, 4B4, 0H4, Leu 8), B cells (CD22), activated cells CD25, OKT9, HLA‐DR), Langerhans cells (CD1) and macrophages (Leu‐M5). The results showed many similarities between LIS and PLE. The most important differences between these conditions and CDLE/SCLE were the high proportions of cells reactive with monoclonal antibody Leu‐8 and the absence of T cells expressing HLA‐DR antigens in LIS and PLE, suggesting absence of local T cell activation in these conditions. The differential diagnostic and pathogenetic aspects of these findings will be

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1990.tb01670.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Angiogenesis is necessary for normal growth, wound healing, and plays a key role in many pathologic processes. A variety of endothelial markers have been used to investigate angiogenesis. Unfortunately, excellent markers for vascular endothelium in human tissues exhibit little or no staining of endothelia in tissues of other animal species, including the pig. We are interested in the hairless Yucatan strain of mini‐pig as an animal model for studying cutaneous wound healing because its skin is histologically and functionally very similar to that of man. Hoping to find a specific marker to identify vascular endothelium in the mini‐pig, we therefore screened a battery of 11 different lectin‐horseradish peroxidase conjugates. Based on specificity and staining intensity,Dolichos biflorusagglutinin (DBA) was chosen from this battery to investigate vascular changes in the healing of cutaneous wounds in the mini‐pig. When compared with routine histologic sections stained with hematoxylin and eosin, blood vessels were much easier to identify in sections stained histochemically with DBA. Lectin histochemistry was particularly useful in investigations of early events in angiogenesis during wound healing when newly derived capillary buds and minute blood vessels were obscured in normal histologic sections by an inflammatory cell infiltrate associated with the healing wound. Ultrastructural lectin cytochemistry revealed staining along the luminal surface and the basolateral plasmalemma of endothelial cells. Histochemical staining with DBA promises to provide a useful method for further investigation of angiogenesis and other vascular phenomena in a variety of normal and pathologic processes using the hairless Yucatan strain of mini‐pig as the ani

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1990.tb01671.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Intravascular papillary endothelial hyperplasia is an interesting endothelial proliferation, the nature of which has aroused some controversy. Five cases were studied by light microscopy and by immunohistochemistry using antibodies to Factor VHI‐related antigen (FVIII‐rAg), ferritin, α1‐antitrypsin, α1‐antichymotrypsin and vimentin and were compared with conventional intravascular organizing thrombi. The results show a similar progression of the immunophenotype of the endothelial cells in both entities: they are initially positive for ferritin, then acquire vimentin positivity and only display FVIII‐rAg positivity in advanced (“mature”) lesions. This suggests that intravascular endothelial hyperplasia is closely related to organizing thrombi and is probably a peculi

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1990.tb01672.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Metaplastic synovial cyst, a recently described entity, is histologically characterized by a cystic cavity lined by metaplastic synovial tissue that is often hyperplastic and protrudes into its lumen as villous structures. Clinically, these lesions resemble suture granuloma and develop at the site of previous surgical trauma. We have studied 4 cases of this unusual entity and have investigated the immunohistochemical profile in an effort to determine the nature and derivation of their lining tissue. One of our cases is unique since the lesion was associated with a basal cell carcinoma. A history of previous surgery was noted in one, and history of trauma in 0 of the 4 cases. Our immunohistochemical observations confirmed a mesenchymal derivation for the „synovial like villous structures” but non‐availability of a specific „synovial” marker does not permit us to conclude with certainty the synovial nature of the metaplastic tissue. Cutaneous metaplastic synovial cysts are unique lesions which deserve attention and should be included in the differential diagnosis of cutane

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1990.tb01673.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

From among 504 histologic specimens of seborrheic keratosis (SK), 09 showed acantholysis, not related to pemphigus, Darier's disease, actinic keratosis or acantholytic squamous cell carcinoma. Acantholysis was found in 04 (03.3%) of the irritated type of SK, almost exclusively in the squamous cell nests showing dyskeratosis and spongiosis between and around squamous eddies or horn cysts. Clinically, 65.5% of the lesions were located on the face and scalp, 00.7% on the trunk and 13.8% on the limbs. It was speculated that dyskeratotic, degenerative changes of the keratinocytes together with spongiosis were responsible for the acantholysis.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1990.tb01674.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Using a rabbit anti‐human liver ferritin antibody, we examined the binding patterns of this reagent in normal skin and observed a unique binding pattern limited to the outermost layer of the eccrine duct. Examination of a variety of sweat gland neoplasms revealed 2 distinct patterns. One was the binding of this antibody to the outermost layer of cells in the epithelial cords of syringoma, producing a characteristic ring when seen in cross‐section. This pattern of binding did not occur in other neoplasms known to be related to the eccrine duct such as dermal duct tumor and eccrine poroma. Only sparse sporadic binding occurred in other eccrine and apocrine neoplasms. A second characteristic binding pattern, not related to that noted in syringoma and diffuse in pattern, was seen in acrospiroma and in a number of adnexal carcinomas. Diffuse ferritin expression has been described in malignant neoplasms in tissues other than skin. Diffuse ferritin staining of certain sweat gland neoplasms may be an indication of biologic activity and potential aggressivity of these neopla

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1990.tb01675.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Dying cells undergo coagulative necrosis or apoptosis. In the skin, apoptosis is known to occur in graft‐versus‐host reactions, in lichen planus, during regression of plane warts and neoplasms, and after physical injury caused by ultraviolet light resulting in sunburn cells. The present study shows that primary skin irritation also causes apoptosis. Mild, or moderate‐to‐con‐siderable, dithranol irritation of healthy uninvolved human skin caused focally coagulative necrosis of keratinocytes and also apoptosis of scattered keratinocytes, i.e. condensation of chromatin and cytosol, clumping of tonofilaments and budding of membrane‐bound cell fragments. These apoptotic cell fragments were engulfed in the epidermis by macrophages. Colloid bodies were detected in the upper dermis and apparently represented non‐phagocytosed apoptotic cell fragments that had dropped down from the epidermis. Dithranol also caused fibrillar degeneration of melanocytes and in some cases of Langerhans' cells, indicating that colloid bodies in the upper dermis could partly derive from these cell types. The significance of apoptosis in irritant contact dermatitis could be to maintain homeostasis of epidermis and counteract the hyperplastic response caused by irritant stimuli. Another possibility is that apoptosis was the response to an injury less severe than that cau

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1990.tb01676.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A clinical and histologic review of 22 patients (13 males, 9 females) with cutaneous lesions classified as desmoplastic trichilemmoma is reported. Typically, the lesions occur as solitary dome‐shaped papules on the face during (at least) the fifth decade of life. The average age was 64 years (range 19–89) with a median age of 66.5 years. The most frequent clinical diagnosis was basal cell carcinoma, and the most common sites were the lip, eyebrow and nose. Two lesions occurred in examples of nevus sebaceus of Jaddasohn. The proportion of desmoplasia varies, but is generally between 20% to 60% of the lesion. Ulceration is seen in a minority of lesions but when present, is closely associated with underlying desmoplasia. Desmoplasia may be seen in small, as well as large, trichilemmomas; it generally occurs centrally but at times may be seen peripherally. The appearance of strands of epithelial cells entrapped in dense fibrosis and hyalinization may mimic desmoplastic variants of squamous cell carcinoma and basal cell carcinoma. This appearance may be particularly troublesome when the desmoplasia occurs at the base of the biopsy specimen. Knowledge of this phenomenon and the search for more typical features of trichilemmoma such as clear cells and peripherally palisaded columnar cells upon a thickened, eosinophilic basement membrane will allow the correct diagnosis to be made. We believe that the desmoplasia occurs as a secondary change in pre‐existing trichilem

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1990.tb01677.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A case of an unusual benign apocrine hamartoma was studied by light microscopic, immunohistochemical, and electron microscopic methods. This tumor clinically showed a linear configuration and was located on the midline chest of a pubescent male. Microscopic studies revealed features of both a tubular apocrine adenoma and a syringocystadenoma papilliferum.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1990.tb01678.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY