摘要:

In the majority of human cells the transferrin receptor (TFR) plays an important role by mediating the cellular iron uptake. The TFR is especially expressed by proliferating cells and has been found in high amounts in malignant tumors. The distribution of the TFR in frozen sections of 89 skin biopsies of normal skin and of various cutaneous tumors was investigated using an anti‐TFR monoclonal antibody (OKT9) and a 3‐step immunoperoxidase method. Our results indicate that in normal human skin the TFR shows a characteristic microanatomical distribution in the basal cell layer correlated to the architectural pattern of the dermal‐epidermal interface. In cutaneous lymphomas of high grade malignancy the TFR was expressed in virtually all cells compared to only 25–75% in low grade lymphomas. Malignant melanomas were strongly positive in the whole tumor tissue, whereas benign melanocytic nevi were largely negative. Obviously the immunohistochemical demonstration of the TFR may serve as prognostic indicator or diagnostic aid, respe

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00119.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The APAAP technique is an unlabelled antibody bridge technique which can be used on either frozen or paraffinembedded sections. One applies first a monoclonal antibody, then a polyclonal bridge antibody, and finally a soluble complex of alkaline phosphatase and monoclonal mouse anti‐alkaline phosphatase. Subsequently, the enzyme label is developed with a naphthol salt and new fuchsia as a dye. This technique was used in our laboratory on frozen and/or paraffin embedded sections by using 15 different monoclonal antibodies, which are commercially available. The reaction product was bright red and could easily be distinguished from the brown color of melanin, which makes the APAAP technique particularly suitable for dermatopatholog

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00120.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Mitotic rates (mitotic figures per 10 400X fields) were determined for 115 sweat gland adenomas. The observations were also combined for all lesions within each category of adenoma. The combined mitotic rates of spiradenoma, cylindroma, syringoma, and chondroid syringoma were each less than 1.0. Individual lesions within these categories often lacked mitotic figures. With the exception of 1 chondroid syringoma, none of the individual lesions within these groups had mitotic rates exceeding 2.0. The combined mitotic rates of acrospiroma and hidradenoma papilliferum were 1.6 and 1.52, respectively. Twenty‐live percent of the acrospiromas had mitotic rates exceeding 2.0 (maximum 7.5). Milotic figures were concentrated in specific cell populations, particularly in acrospiromas and chondroid syringomas. The potential for cell division appeared to be reduced or lost in areas of increased cytologic or structural differentiation. A review of the literature indicates that there can be overlap between the mitotic rates of sweat gland adenomas and those of carcinoma

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00121.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Malignant transformation occurred in pre‐existing sweat gland tumors in 7 patients. Three lesions showed an histologic pattern of eccrine spiradenoma, 2 eccrine poroma, one cylindroma and one papillary eccrine adenoma. Malignant transformation was histologically characterized by the presence of solid tumor areas populated with large cells having irregularly shaped nuclei and mitotic figures. There were multiple foci of squamous metaplasia, areas of loss of basement membrane and invasion of the surrounding connective tissu

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00122.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Several Moll's gland cysts from 4 different patients were studied with the light and electron microscopes. The cysts were lined with a combination of several different epithelia, such as completely keralinized epithelium, glycogen‐rich, stratified squamous epithelium with incompletely keratinized luminal cells, sweat duct epithelium, and secretory cells. In one case in addition to the above components, secretory‐type wall cells contained very large lamellar inclusions which were PAS positive and diastase resistant. It was concluded that Moll's gland cyst is composed of dilated duct of the Moll's gland and secretory segment; the proportion of each segment is variable but the portion showing ductal differentiation is usually predominant and typical secretory epithelium is not always s

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00123.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Granular cell basal cell carcinoma (BCC) is a rare histologic variant of BCC. In this, the third reported case, the tumor consisted almost entirely of granular cells. By electron microscopy, these cells were filled with pleomorphic lysosome‐like granules lined by unit membranes and containing homogeneous or granular electron‐dense bodies, membranous debris and amorphous material. The epithelial origin of the tumor was suggested by the characteristic light microscopic appearance of tumor cell islands with some areas of peripheral palisading, and was confirmed by electron microscopic features of numerous well‐formed pentalaminate desmosome junctions and sparse cytoplasmic tonofilament bundles, some of which showed attachments to the desmosomes. Histochemical immunoperoxidase stains for lyso‐zyme showed no cytoplasmic lysozyme activity, in common with other granular cell tumors, but ultrastructural examination did not reveal angulate bodies, in contrast to findings in non‐epithelial granular ce

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00124.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

A 60‐year‐old woman developed a progressive linear pigmentation on the trunk. Skin biopsy demonstrated an increase in the number and size of neural fibers in the dermis. Clinical and paraclinical investigations of this unusual disease showed findings similar to the hereditary type of Sipple syndrome (multiple endocrine neoplasia syndrome, Type 2b). Such findings as Marfanoid habitus, abnormal electromyography and hypertrophy of the conical nerves suggest that our patient's disease and Sip‐pie syndrome are identical. However, such other symptoms of Sipple syndrome as presence of endocrine tumors are absent. Questions of long‐term prognosis and physiopathogenicity are raised, since no nerve growth factor was detected in this patient. The name of linear cutaneous neuromas is suggested for the patient's lesions, and a possible association with the pigmentation and cutaneous neuromas of the MEN 2b s

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00125.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

We report a case of a long‐standing subcutaneous nodule of the scalp in a young man, with the histological features of a primary cutaneous meningioma. The results of an immunohistological study using a panel of monoclonal antibodies gave a similar pattern to that seen in intracranial meningiomas, and distinct from other cutaneous lesions which may be considered in the differential diagnosi

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00126.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

We describe a patient who in association with myeloma developed generalized keratotic horns. Light microscopy revealed parakeratotie plugs resembling those seen in Kyrle's disease. Electron microscopy showed distortion of keratinosomes and laminated bodies indicating a profund disturbance in keratinization. A strong C3and weaker IgG and IgA immunofluoresence were seen in the areas of epidermal plugs and horns but no circulating epidermal antibodies were detected. All symptoms disappeared when therapy for myeloma was started favoring that (hey were of paraneoplastic origin.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00127.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Macromelanosomes in a spindle and epithelioid cell nevus, which occurred in the electron beam‐damaged skin of a woman with mycosis fungoides, were ultrastructurally investigated. The melanosomes in the spindle and epithelioid cells were varied in size but uniformly spherical in shape and composed of a core and cortex structure. Electron‐lucent bodies were observed in the small melanosomes as well as in the macromelanosomes. Positive dopaoxidase reaction was revealed on the cortex of these melanosomes and partly on their electron‐lucent bodies. The electron‐lucent bodies were not vesicular structures but were aggregates of filamentous elements, which had a continuity with fibrous structures in the melanosome cortex. It is concluded that the electron‐lucent bodies may be one of the matrices of melanization. Anomalously large accumulations of these bodies and concentrical melanization seem to lead to the formation of macrome

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00128.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY