摘要:

Epidermal keratinocytes separated from skin lesions of non‐bullous congenital ichthyosiform erythroderma were investigated in an attempt at experimental reproduction of this keratinization disorder.In vitrostudies on growth and differentiation of pathological keratinocytes isolated from the influence of the host's dermal and humoral components were performed using the immersed epidermal cell culture technique. Ten to 25‐ day‐old confluent and stratified cultures were examined by means of photonic and electron microscopy, and stained with various differentiation markers for indirect immunofluorescence studies. The cultured epidermis showed low‐grade differentiation and no clear‐cut evenly distributed signs of the original disease. Grafting on congenitally athymic nude mice allowed further differentiation of the epidermal sheets and re‐expression of the histologic and ultrastructural features of non‐bullous congenital ichthyosiform erythroderma. Thus, the purely epidermal origin of this particular form of autosomal recessive ichthyosis could be confirmed. Large amounts of pathological keratinocytes generated from small skin biopsies may be used for experimental purposes after grafting on several at

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1989.tb00001.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Morphometry was carried out on H&E stained paraffin sections of 29 cases of contact dermatitis (CD) and 35 cases of mycosis fungoides (MF) (patch stage 12; plaque stage 11; tumor stage 12); 9 nuclear parameters, mean thickness of the infiltrate and 5 stereological parameters were assessed for each slide. Application of a non‐parametric discriminant analysis (k‐nearest neighbour method) which is based on median of nuclear areas, mean maximal nuclear diameter, volume density and numerical density of nuclei provided discrimination between CD and patch stage MF at an efficiency of 82.9% (specifity 86.7%, sensitivity 61.5%). Efficiency of discrimination between CD and plaque stage was 92.5% (specifity 89.7%, sensitivity 91.7%) and between CD and tumor stage 100% when discriminant analysis was based on the mean thickness of the infiltrate. Although unequivocal discrimination between CD and MF cannot be achieved in each individual case, morphometry on routine paraffin material obviously provides additional objective criteria for the diagnosis of early

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1989.tb00002.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1989.tb00003.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

A hair matrix tumor showing an unusual tumor cell arrangement was found at the base of a solitary trichoepithelioma. Coexisting with solid epithelial islands and immature hair follicle‐like structures, areas of alternating epithelial cords and hand‐like stroma resembling the Verocay bodies of neurilemmoma or “ripplemarks” on waves were found. In other areas myxomatous degeneration of the stroma changed the rippling into a cribriform pattern. In some parts of the tumor there was a dense melanin pigment associated with MEL5 stained melanocytes. S‐100 and GDI (OKT6) antigen stains demonstrated Langerhans cells scattered in the parenchyma and less frequently in the stroma. The majority of tumor cells were considered immature pilar cortical cells because of the following: 1. HKN‐6 was strongly positive; 2. a large number of melanocytes were associated with tumor cells in some foci; 3. ultrastructurally immature tumor cells, which had electron‐dense tonofilaments and many desmosomes, were transformed without production of trichohyalin granules into semikeratinized cells which showed nuclear degeneration and loss of electron density in tonofilaments. This tumor, however, has not attained the degree of differentiation observed in trichoblastoma (1) another example of an immature cortical cell tumor. Squamous eddy‐like or horn pearl‐like foci of incomplete keratinization and large keratin‐filled cysts were also present within the immature parenchyma, indicating that some immature cells were differentiating toward non‐cortical cells, as found in the outer sheath. We would like to designate this tumor “rippled pattern trichom

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1989.tb00004.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

We report the light and electron microscopic findings of an unusual, locally aggressive cutaneous clear cell carcinoma involving extensively the left cheek and upper lip of a 61–year‐old woman. The patient remained free of recurrence 5 years after wide local excision. Microscopically, the tumor was a poorly circumscribed and deeply invasive clear cell neoplasm characterized by large lobules and convoluted trabeculae of polygonal and columnar cells with palisading of the peripheral cells rimmed by thick basement membranes, and horn microcysts with tricholemmal keratinization. The tumor infiltrated the muscle and the salivary glands. Only slight cytologic atypia and a few mitotic figures were present. No definite ductal differentiation was found. Immunoperoxidase staining for carcinoembryonic and epithelial membrane antigens was negative. Ultrastructurally, the tumor cells were joined by desmosomes, and surrounded by thick basal lamina. The cytoplasm contained large pools of glycogen. Peculiar whorls of tonofilaments and aggregates of needle‐shaped and club‐shaped structures were observed in a small keratinizing focus. The tumor showed features resembling the outer root sheath of the hair follicle microscopically. Furthermore, the lack of duct‐like structures and immunoreactivity of carcinoembryonic antigen tend to favor tricholemmal over sweat gland differentiation in this tumor which possibly represents a rare example of tricholemmal

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1989.tb00005.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The clinical and pathological features of 4 cases of pilomatricoma associated with epidermoid cysts, are described. None of the cases showed features of Gardner's syndrome.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1989.tb00006.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Focal acantholytic dyskeratosis (FAD) is considered an incidental histological finding of unknown etiology. It has been described in association with only few pathological conditions. To the best of our knowledge, we report the first case of focal acantholytic dyskeratosis occurring in condyloma acuminata.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1989.tb00007.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1989.tb00009.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY