ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05681.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05805.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05806.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05807.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05808.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

ABSTRACTIn this study, the presence of actin in cultured trypanosomatids was investigated using polyclonal antibodies to heterologous actin. Polyclonal antisera to rabbit muscle actin and a monospecific anti‐actin antibody react with a 43‐kDa polypeptide in extracts ofTrypanosoma cruzi, Herpetomonas samuelpessoaiandLeishmania mexicana amazonensison protein immunoblots. The 43‐kDa polypeptide co‐migrates with skeletal muscle actin and is retained within trypanosomatid cytoskeletons. Attempts to isolateH. samuelpessoaiactin through DNase I affinity chromatography showed that the 43‐kDa polypeptide did not bind to the column. Instead, low yields of a 47‐kDa polypeptide were obtained indicating that the trypanosomatid actin displays unusual DNase I binding behavior when compared to actins from higher eukaryotes. Immunofluorescence studies confirmed that cytoskeletons retain the actin‐like protein. InH. samuelpessoai, actin is localized in the region close to the flagellum, whereas inT. cruziit is more homogeneously distributed. The data presented here show that trypanosomatid actin displays biochemical characteristics similar to actins of o

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb02666.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

ABSTRACT.Large numbers ofPneumocystis carinii(2 × 1010nuclei) were isolated and separated from the lungs of immunosuppressed rats by an enzymatic (collagenase, hyaluronidase and DNase) digestion procedure. The nucleic acid isolated from thisP.carinnii‐enriched preparation was characterized by melting point analysis and RNA‐sizing gels. The GC content ofP. cariniiDNA was approximately 33% while the rat DNA was 41.4%. In addition, RNA isolated from theP. carinii‐enrichedpreparation showed unique ribosomal RNA bands of 3.4 kb and 1.8 kb as compared with uninfected rat lung ribosomal RNA which banded at 4.8 and 1.9 kb. Following isolation and fragmentation by sonication, theP. cariniiDNA fragments were inserted into the vector, λ gt‐11. The resultant library contained 1.1 × 105phage, of which 40–45% hybridized toP. cariniiDNA but no

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05809.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

ABSTRACT.Total RNA fromPneumocystis cariniiobtained directly from the rat lung and from short term culture on A549 cells was evaluated for size and purity. An isolation procedure using guanidine isothiocyanate and lithium chloride was preferable to a hot phenol method. Host cells were eliminated by hypotonic lysis and a series of microfiltrations.Pneumocystis cariniiwere pretreated with Zymolyase for increased susceptibility to chaotropic agents. The major ribosomal species ofP. cariniiRNA migrated similarly toSaccharomyces cerevisiaerRNA. The 28s‐like species migrated well ahead of rat and A549 cell rRNA and well behind the prokaryotic large rRNA specie

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05810.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05811.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe cytoplasmic 5S ribosomal RNA sequence fromPneumocystis cariniiwas determined and compared with those of 382 eukaryotes and an evolutionary tree was constructed to establish the phylogenetic position ofPneumocystis.The data suggest thatPneumocystisis associated with the Rhizopoda/Myxomycota/ Zygomycota group but not with common fungi, such as Ascomycota or Basidiomycota, nor with other protozoa.

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05812.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY