摘要:

Previous studies on the frequency of intestinal mast cells and eosinophils in patients with inflammatory bowel disease yielded conflicting results. In the present morphometric study, we quantified mast cells and eosinophils in the lamina propria by histological and immunohistochemical methods in 64 patients suffering from Crohn’s disease (33 cases) or ulcerative colitis (31 cases), and in 29 controls. Histological data from 206 biopsies were related to the presence of mucosal inflammation and clinical parameters. The number of eosinophils was increased in patients with inflammatory bowel conditions (mean ± SE: 331 ± 44/mm2) as compared to controls (258 ± 27/mm2), and was dependent on disease activity and drug treatment. Mean mast cell numbers did not differ between patients and controls. However, a reduced mast cell number was found in toluidine blue‐stained sections of actively inflamed tissue areas (143 ± 16/mm2, versus 206 ± 18/mm2in non‐inflamed tissue). Immunohistochemical studies using antibodies against the granule proteins tryptase and chymase suggest that this decrease in mast cell numbers is due to mast cell degranulation. The present data show that the number of intestinal mast cells and eosinophils is altered in patients with inflammatory bowel diseases, suggesting that both cell types are involved in the pathogenesis of c

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.262309.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

T‐cell rich B‐cell non‐Hodgkin's lymphoma (T‐cell rich B‐cell lymphoma) is a morphological variant of diffuse large B‐cell lymphoma. It is important to recognize this variant in the differential diagnosis of T‐cell non‐Hodgkin's lymphoma. The main differential diagnosis of T‐cell rich B‐cell lymphoma, nodular and diffuse lymphocyte predominance Hodgkin's disease (lymphocyte predominance Hodgkin's disease), is, however, even more difficult and differentiating criteria are still not satisfactorily defined. Moreover, T‐cell rich B‐cell lymphoma may not represent a clinicopathological entity. Twelve cases of T‐cell rich B‐cell lymphoma, selected on the basis of morphology and limited immunohistochemistry without previous knowledge of clinical data, were studied by immunohistochemistry and polymerase chain reaction for bcl‐2 rearrangements to investigate the histogenetic background. In three of 12 cases, bcl‐2 rearrangements were found, strongly suggesting a follicle centre cell origin. In three other cases, a documented history of definite nodular lymphocyte predominance Hodgkin's disease 29 months to 20 years prior to the diagnosis of the lymphoma was present. No differences in growth pattern, residual nodularity, tumour cell distribution, cellular morphology and composition, or immunophenotypical differences were noted in these six cases as compared to the remaining cases. These data underscore the histogenetic diversity in T‐cell rich B‐cell lymphoma and identify it as a progressed form of lymphoma derived from entities as diverse as follicle centre cell lymphoma and nodular lymphocyte predominance Hodgkin's disease. Moreover, it shows a complete morphological overlap with the diffuse form of lymphocyte predominance Hodgkin's disease and may act

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.265306.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

During a 9‐year‐period, 50 cases of primary non‐Hodgkin's lymphoma (NHL) of the thyroid gland were reported to a population‐based lymphoma registry covering western Denmark giving an incidence of 2.06×10−6cases per year. The male:female ratio was 1:4, and the mean age was 72.8 years for women and 62.8 years for men. On histomorphological reclassification 83% of the cases showed a high grade and 17% a low grade morphology. 98% had a B‐phenotype and 2% a T‐phenotype. In at least 33 of the cases, primary NHL of the thyroid gland was preceded by Hashimoto's thyroiditis and at least 25 of the patients had a high grade NHL which was transformed from Hashimoto's thyroiditis through a low grade B‐cell lymphoma of MALT type. The most frequent presenting symptoms were goitre (100%), hoarseness (57%), stridor/dyspnoea (55%) and dysphagia (45%); thirty‐six percent of the patients were hypothyroid at the time of diagnosis. Seventy‐six percent of the patients had localized disease (stages 1–2) and 24% had disseminated lymphoma (stages 3–4). Five year survival was 34.5% and 5 year cause‐specific survival 46.2%. The following factors were associated with a poor prognosis: stage 3–4 disease, elevated S‐urate, presence of hoarseness and age>66 years. Morphological subtype did not correla

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.268311.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

Signet‐ring cell carcinoma of the urinary bladder is a rare tumour, accounting for approximately 0.24% of all bladder malignancies. In this study, the clinicopathological findings in 13 cases are described. This malignancy is far more common in men than in women (ratio 11:2). The distribution by age and clinical symptoms can not distinguish it from transitional cell carcinoma. The tumour behaves like other high grade malignancies, presenting frequently at an advanced stage, and having an unfavourable clinical outcome. No special therapy seems superior to anothe

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.262303.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

Histiocytic necrotizing lymphadenitis, also called Kikuchi‐Fujimoto (KF) disease, is a benign disorder characterized histologically by paracortical necrotic foci surrounded by histiocytic aggregates. We analysed affected lymph node tissues from 34 patients with the disease in an attempt to elucidate its histogenesis. The ‘necrotizing’ cells showed typical apoptotic changes, including cell shrinkage and condensed and fragmented nuclei. Apoptotic bodies with a peculiar ultrastructure were demonstrated, and DNA fragmentation was detected in these cells by in situ end labelling. Immunostaining for the apoptosis‐regulating proteins bcl‐2, bax, c‐myc and p53 failed to show their involvement in KF disease. However, perforin, a killer cell‐specific cytolytic protein essential for provoking apoptosis in target cells, was found to be expressed abundantly by the infiltrating cells, which were thought to be cytotoxic T‐lymphocytes. Perforin‐expressing cells were present in the apoptotic foci of 28 of the 34 patients (82.4%). Virtually no cells containing perforin granules were present in non‐pathological regions, lymph node tissues from control subjects with reactive or tuberculous lymphadenitis or those from patients with KF disease with negligible apoptosis. Therefore, the ‘necrosis’ associated with KF disease appears to be attributable totransapoptotic death of the killer cell target in the affected nodes. We propose that KF disease should be called

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.267310.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

Wilms’ tumour (nephroblastoma) has been associated with chromosomal abnormalities at the 11p13, 11p15 and 16q regions. A study into the possibility of mutations occurring within p53, the ubiquitous adult tumour suppressor gene, in Wilms’ tumour was carried out. Thirty‐eight ca ses were studied. Of these 36 were categorised into the favourable histology group and two into the unfavourable histology group based on the National Wilms’ Tumour Study criteria. Archival formalin‐fixed, paraffin‐embedded tissue sections from each case were stained with a polyclonal (AB565:Chemicon) and a monoclonal (DO7:Dako) antibody raised against p53 protein using a peroxidase‐labelled streptavidin biotin kit (Dako). ‘Cure’ (disease‐free survival of 60 months or longer) was documente d in 39% of cases with favourable histology tumours. Eleven percent in this group succumbed to the disease. Both cases with unfavourable histology died. Four out of 36 (11%) tumours with favourable histology demonstrated weak to moderate staining with both AB565 and DO7 in more than 75% of tumour cells. In contrast, p53 protein expression in unfavourable histology tumours was significantly increased compared with the favourable histology group (P = 0.021) with both cases demons trating immunopositivity in>75% of tumour cells when stained with AB565 and DO7. The intensity of staining ranged from moderate to strong in both cases. It appears from this preliminary study that the immunohistochemical expression of p53 protein in Wilms’ tumour, presumably a result of mutation in the p53 tumour suppressor gene, correlates with histological classification, histological categorisation being one of the useful features in the prognostic ass

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-384.x

出版商:Blackwell Science    

年代:1996

数据来源: WILEY

摘要:

Using a polyclonal pan‐cadherin antibody and a monoclonal E‐cadherin antibody (HECD‐1) we have investigated cadherin expression in lymphomas and reactive lymph nodes. Routinely processed tissue from nine reactive lymph nodes and 48 lymphomas (six T‐cell, six high‐grade B‐cell, 15 low‐grade B‐cell, 13 anaplastic large cell and eight Hodgkin’s disease) were immunostained. The reactive cases showed pan‐cadherin membrane associated staining of endothelium and epithelioid granulomas. No staining of lymphoid cells was seen. Pan‐cadherin immunostaining was present in three of six T‐cell lymphomas, two of six high‐grade B‐cell lymphomas, 12 of 13 anaplastic large cell lymphomas and three of eight cases of Hodgkin’s disease. No staining of low‐grade B‐cell lymphomas was identified with the pan‐cadherin antibody. E‐cadherin was not detected in any of the lymphomas that showed pan‐cadherin expression. The frequent and strongest cadherin expression in anaplastic large cell is noteworthy. The tumour cells of this lymphoma subtype are characterized by copious cytoplasm and a cohesive appearance, features which impart a superficial resemblance to carcinoma cells. Since cadherin molecules are known to have major morpho‐regulatory functions our data suggests that the expression of cadherin molecules by anaplastic large cell lymphomas may play an important role in determining t

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.266305.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

To investigate the pattern and prevalence of membranous fat necrosis of the breast we examined 25 breast biopsies or mastectomy specimens performed for recurrent lumps following conservative surgery and radiation therapy. In addition, we examined 41 consecutive cases taken from our files coded as fat necrosis which were associated with several aetiologies. Our results demonstrate that membranous fat necrosis of the breast occurs in association with several aetiologies but in its florid form is most often due to radiation therapy.

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.252292.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

We retrospectively reviewed 107 consecutive cases of radial scar and complex sclerosing lesions in a 54 month period seeking vascular lesions using archival H&E stained 5 μm thick sections. Eight showed vascular abnormalities, five being false aneurysms which were limited to 62 lesions>10 mm diameter; no false aneurysm was found in 45 radial scars ≤10 mm. All cases displayed arterial lesions, but two also showed associated venous fibrosis. Traumatic false aneurysms with loss or severe thinning of the media and intimal, medial or extravascular spindle cell proliferation were found in the arteries of five cases with histological evidence of local reaction to previous fine‐needle aspirational biopsy and clinical documentation of aspiration 18 to 121 days before. The diameters of the false aneurysms correlated with the original widths of the arteries. The other lesions with mature intimal fibrosis in arteries or veins lacked such local post‐aspiration expansile reactive changes, or a history of fine needle aspiration. We conclude that traumatic false aneurysms found in complex sclerosing lesions seem to be iatrogenic, resulting from fine needle aspiration. Mature fibrosis in veins or arteries are probably unrelat

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.273312.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

We report a Chinese patient who presented with metastatic undifferentiated carcinoma in the cervical lymph node of unknown primary origin. Despite a raised IgA titre against Epstein‐Barr virus capsid antigen, examination and biopsy of the nasopharynx were negative. Radiotherapy was given to the head and neck region with the orbit shielded. There was complete resolution of the metastatic lymph nodes. She developed proptosis of her left eye one year afterward when a large tumour was found in the lacrimal sac region. Review of the initial computerized tomography revealed a small soft tissue mass in the same region. The tumour was composed of undifferentiated carcinoma cells associated with dense lymphoid infiltrate. In situ hybridization for Epstein‐Barr virus EBER RNA showed strong positive signals in the malignant cells. This is the first reported case of an Epstein‐Barr virus positive undifferentiated carcinoma with lymphoid stroma in the lacrimal sac. In addition to the nasopharynx, salivary glands, nasal cavity and paranasal sinuses, the lacrimal sac should be considered as a potential primary site for Epstein‐Barr virus positive metastatic undifferentiated carcinoma in the cervical lymp

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.264307.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY