摘要:

Studies on the vital staining of bacteria were carried out to determine the concentrations of tetrazolium salts required for optimal staining, and the amounts which could cause complete inhibition of bacterial growth. Four salts were studied: neotetrazolium chloride (NTC), triphenyltetrazolium chloride (TTC), blue tetrazolium (BT), and nitro-blue tetrazolium (NBT). Evidence has been presented to show that only living, actively metabolizing cells can reduce neotetrazolium to formazan.

ISSN:0008-4166    

DOI:10.1139/m59-028

出版商:NRC Research Press    

年代:1959

数据来源: NRC

摘要:

An opinion is offered to assign "Klebsiella", which are nonmotile, indole positive, and generally gelatinolytic, to the Oxytoca group. The biochemical reactions of 19 strains of the Oxytoca group are described by methods recommended by the Enterobacteriaceae Subcommittee of the Nomenclature Committee of the International Association of Microbiological Societies for group differentiation within the Enterobacteriaceae. Fifteen of these strains were isolated from the oropharyngeal region of patients with disease.

ISSN:0008-4166    

DOI:10.1139/m59-029

出版商:NRC Research Press    

年代:1959

数据来源: NRC

摘要:

Eight bacteria capable of decomposing 2,2-dichloropropionate (dalapon) were isolated from soil by means of enrichment techniques and selective media. The decomposition was demonstrated by the clearing of a solid medium containing mineral salts, dalapon, and CaCO3; by a lowering of the pH of a liquid medium containing dalapon as the carbon source; by the increase in chloride ion in the liquid medium; and by the consumption of oxygen by three of the isolates when dalapon was the sole carbon source. Six of these were tentatively classified asAgrobacteriumand two were tentatively classified asPseudomonas, although there was much overlapping of characteristics. These organisms and many unidentified actinomycetes, molds, and bacteria, including aMicrococcusspecies, overcame the inhibitory effect of dalapon on an agar-decomposing bacterium when grown on the same plate.

ISSN:0008-4166    

DOI:10.1139/m59-030

出版商:NRC Research Press    

年代:1959

数据来源: NRC

摘要:

The growth-promoting action ofC.albicanscells on tubercle bacilli is due, in all probability, to a polysaccharide fraction which has been isolated from yeast cells. A technique for isolating this fraction, some of its chemical characteristics, and its action on tubercle bacilli have been described.

ISSN:0008-4166    

DOI:10.1139/m59-031

出版商:NRC Research Press    

年代:1959

数据来源: NRC

摘要:

Cultures ofSerraliaeven when unpigmented can be identified by their flagella which have an unusual coiled shape.Serratiacultures isolated from clinical materials are lactose negative, imvic − − + +, sulphide negative, anerogenic, and have a distinctive pattern of fermentative abilities. A differentSerratia, not so far encountered in clinical materials, is also described. The literature on isolation ofSerraliafrom man and animals is summarized.

ISSN:0008-4166    

DOI:10.1139/m59-032

出版商:NRC Research Press    

年代:1959

数据来源: NRC

摘要:

A specific and adaptive 2-keto-D-gluconokinase has been isolated from cells ofLeuconostoc mesenteroidesgrown on 2-keto-D-gluconate. The enzyme, purified 138-fold, has an optimum pH of 7.7, is most stable between pH 6.0 and 8.0, and is quickly inactivated at temperatures above 40 °C. Magnesium chloride is required for activity. ATP, ITP, and GTP served as phosphate donors but ADP and UTP were inactive. Whereas EDTA and glycine stabilized the enzyme,p-chloromercuribenzoate, hydroxylamine, copper acetate, mercury acetate, and sodium fluoride were inhibitory. The product of the enzymatic reaction was isolated and characterized as 2-keto-6-phospho-D-gluconate.

ISSN:0008-4166    

DOI:10.1139/m59-033

出版商:NRC Research Press    

年代:1959

数据来源: NRC

摘要:

Growth curves forB.slearothermophiluswere established for temperatures between 45 °C and 70 °C. Generation times as short as 10 minutes were obtained. At 65 °C initial logarithmic growth was followed by autolysis of over 90% of the ceils and subsequently by initiation of a second period of logarithmic growth. The effects of temperatures from 45 °C to 64 °C on the characteristics of the growth curves are almost identical with changes reported forE.colibetween 22 °C and 42 °C.

ISSN:0008-4166    

DOI:10.1139/m59-034

出版商:NRC Research Press    

年代:1959

数据来源: NRC

摘要:

The fluorescent pigments elaborated in cultures of certain species ofAzotobacterwere compared with riboflavin produced by the same cultures. No correlation between the amounts of fluorescence and riboflavin was observed. Iron appears to inhibit the synthesis of the fluorescent pigments but stimulates riboflavin production. The fluorescent pigments obtained fromAzotobacterwere water-soluble, thermostable, insoluble in water-free organic solvents, and each exhibited a single absorption peak in the region of 370–380 mμ in slightly acid solution shifting to 400–420 in alkaline solution. Partial purification has been achieved by continuous paper electrophoresis with an acid electrolyte. These studies appear to indicate that the marked fluorescence observed in certainAzotobactercultures cannot be attributed to riboflavin.

ISSN:0008-4166    

DOI:10.1139/m59-035

出版商:NRC Research Press    

年代:1959

数据来源: NRC

摘要:

The original Japanese observations on kanamycin are confirmed as to activity in vitro and in mice. They are extended to include: (a) cross-resistance studies, (b) experiments with kanamycin A and B singly and together, (c) combined therapy experiments with other tuberculostatic agents.Samples of kanamycin A and A plus B were of the same order of activity as streptomycin in vitro and in mice, and were effective against streptomycin-resistant organisms. Kanamycin B did not increase mouse survival when tested at a level that with kanamycin A gave good prolongation of life. When mice were treated with combinations of kanamycin plus streptomycin, or dihydrostreptomycin, or neomycin, the survival increases obtained were greater than were to be expected from the mere sum of the effects of the individual antibiotics.

ISSN:0008-4166    

DOI:10.1139/m59-036

出版商:NRC Research Press    

年代:1959

数据来源: NRC

ISSN:0008-4166    

DOI:10.1139/m59-037

出版商:NRC Research Press    

年代:1959

数据来源: NRC