摘要:

A new growth factor forMycobacterium tuberculosisis described. It is produced byCandida albicansand stimulates the growth of tubercle bacilli of reduced viability or multiplication rate, as this is observed after treatment of the patient by chemotherapeutic or antibiotic agents. A method for the earlier detection ofM.tuberculosisgrowing on Loewenstein's medium is described: Loewenstein's culture media, previously inoculated with the pathological specimen suspected to contain tubercle bacilli, are superinoculated with suspensions ofCandida albicanswhose dependence upon the presence of tubercle bacilli to grow on this medium has been enhanced. Colonies ofCandida albicanswill "trace" the presence ofM.tuberculosis.

ISSN:0008-4166    

DOI:10.1139/m55-012

出版商:NRC Research Press    

年代:1954

数据来源: NRC

摘要:

Evidence is offered for the existence of several cellulolytic components in the extracellular filtrates of microorganisms. Components separated chromatographically differ in their relative activities on native cotton, swollen cellulose, and CMC. A synergistic effect was obtained when separated components were recombined.

ISSN:0008-4166    

DOI:10.1139/m55-013

出版商:NRC Research Press    

年代:1954

数据来源: NRC

摘要:

A strain ofClostridium botulinumType E in a beef heart infusion – peptone broth yields moderate amounts of toxin when grown at 30 °C. but fails to produce toxin at 37°. When grown in a cellophane sac suspended in a similar medium, at 30 °C, there is a 5- to 10-fold increase in the yield of toxin. Toxoid has been prepared from the high potency cellophane produced toxin by clarification with charcoal, Mandler filtration, and detoxification with 0.3% formalin at 30 °C. for 20 days. Three doses of the fluid toxoid protects mice against 500 M.L.D. of homologous toxin. One dose of the same toxoid adsorbed on alum affords the same level of protection as three doses of the fluid toxoid. Mixing Type E toxoid with equal amounts of Types A and Bin vitroaugments the antigenic action of the Type E toxoid.

ISSN:0008-4166    

DOI:10.1139/m55-014

出版商:NRC Research Press    

年代:1954

数据来源: NRC

摘要:

A Gram-negative rod, conforming; toPseudomonas viscosa(Frankland and Frankland) Migula, was isolated in practically pure culture from a sample of wheat that did not show the usual mixture of epiphytes.In vitrostudies revealed an unusual antibiotic spectrum against a variety of Gram-positive and -negative bacteria as well as againstHelminthosporium sativumandFusarium culmorum. Comparative.studies of the organism andP.aeruginosa,P.fluorescens, andP.chlororaphisindicated that it possessed greater antibiotic activity than any of these three species ofPseudomonas. Application to the control of some plant pathogenic organisms is suggested.

ISSN:0008-4166    

DOI:10.1139/m55-015

出版商:NRC Research Press    

年代:1954

数据来源: NRC

摘要:

A procedure for the isolation, of pentose nucleic acid (PNA) fromPseudomonas hydrophilahas been outlined. The purified PNA is free from protein and desoxypentose nucleic acid (DNA). The pentose component of the PNA has been identified as ribose by conversion to itsp-bromphenylhydrazone. Adenine, guanine, cytosine, and uracil were found in molar ratios of 1.00, 1.55, 0.91, and 0.55 respectively.

ISSN:0008-4166    

DOI:10.1139/m55-016

出版商:NRC Research Press    

年代:1954

数据来源: NRC

摘要:

A water soluble pentosan extracted from wheat flour was purified by precipitation with ethanol and treatment with pancreatin. Such preparations contained 78 to 82% pentosan and 0.5 to 1% protein. The pentosan of "squeegee" starch also was obtained by removing the starch with pancreatin. The product contained 60 to 75% pentosan and 1 to 3% protein. A number of fungi, streptomycetes, and bacteria produced extracellular enzymes that hydrolyzed the water soluble pentosan. Some of these were tested against the pentosan of "squeegee" starch and found to attack it also. The fungi and streptomycetes possessed adaptive pentosanases whereas 40% of the active bacteria had constitutive pentosanases. Xylose and, to a lesser extent, arabinose stimulated production of the adaptive pentosanases of the molds and streptomycetes while xylose, but not arabinose, was effective with the bacilli. The more active genera wereAlternaria,Aspergillus,Fusarium,Trichothecium,Trichoderma, andBacillus.

ISSN:0008-4166    

DOI:10.1139/m55-017

出版商:NRC Research Press    

年代:1954

数据来源: NRC

摘要:

Washed and dried cultures ofActinomyces bovis,A.israeli,Nocardia asteroides,Streptomyces albus,S.griseus, andMicromonospora chalceawere treated as described by Scholl (1908). Neither chitin nor cellulose, which form the framework of the Eumycetes, could be isolated. Therefore, from this chemical point of view the Actinomycetales have nothing in common with the true fungi, but rather with bacteria. These findings are in complete agreement with the morphological features of the Actinomycetales which indicate their place among the bacteria.

ISSN:0008-4166    

DOI:10.1139/m55-018

出版商:NRC Research Press    

年代:1954

数据来源: NRC