ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1996.tb00919.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1996.tb00920.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1996.tb00921.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1996.tb00922.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SummarySeveral recent reports have suggested that the sperm counts of normal men are declining in most countries. In this study the sperm counts of Finnish men, and their possible changes during the past 28 years, were investigated. The material consisted of semen samples from 238 normal healthy men of unknown fertility and 5481 men from infertile couples. The means (medians) of semen volume, sperm density and total sperm count in normal men were 3.3 (3.0) ml, 133.9 (94.0) × 106/ml and 396.6 (309.0) × 106, respectively. These parameters and the relative frequency distribution of the sperm density were similar to those reported elsewhere in the 1940s. Multiple linear regression analysis revealed a significant decrease in semen volume, whereas sperm density and total sperm count of infertile men had not changed significantly during the past 28 years. In addition, no change in sperm counts was associated with the year of men's birt

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1996.tb00427.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1996.tb00923.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1996.tb00924.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1996.tb00925.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1996.tb00926.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SummaryMature mammalian spermatozoa have a compact and stable nuclear structure conferred by protamines instead of histones, which are present in all other cellular types. Chromomycin A3(CMA3) is a useful tool for the detection of protamine deficiency in sperm chromatin. The purpose of this study was to correlate the percentage of spermatozoa staining positively for CMA3with sperm parameters and in‐vitro fertilization of human oocytes. Spermatozoa were collected from 56 fertile and 18 infertile men, and washed twice in PBS, fixed in two changes of methanol: acetic acid (3: 1 v: v) spread on rinsed slides treated with APES and dried. Twenty‐four of the semen samples were subjected to both Percoll and swim‐up, and were stained subsequently with CMA3. CMA3‐stained spermatozoa were expressed as a percentage in a count of 200 spermatozoa. A substantial variation in the percentage of CMA3‐stained cells was observed in ejaculated human spermatozoa, varying between 8% and 77%. A strong negative correlation (r= ‐0.64,p<0.001) was found between sperm count and the percentage of CMA3‐stained spermatozoa. No correlation was found between CMA3‐stained spermatozoa and their motility, while excessive sperm morphological abnormalities were related positively to CMA3‐staining. Spermatozoa in samples exhibiting low (8–62%) CMA3‐staining had significantly higher fertilizing rates in vitro than did samples exhibiting high (49–77%) CMA3‐staining. The mean percentage of CMA3‐stained spermatozoa after swim‐up or Percoll preparation (26% vs 31%) did not differ significantly. These results demonstrate a close relationship between CMA3‐staining, fertilization and sperm count, and suggest potential application of this marker for the prediction of sperm q

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1996.tb00429.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY