摘要:

AbstractTemperature and pH significantly affect the growth and oospore production of the test fungi. The optimum temperature for mycelial production was nearly the same on both solid and liquid media. H‐ion concentration has milder effect than temperature. The optimum temperature and pH value for oospore production agree remarkably with their respectives for growt

ISSN:0044-2208    

DOI:10.1002/jobm.19830230102

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

摘要:

AbstractFermentations for the overproduction of citrate and isocitrate withS. lipolyticain media containing both glucose andn‐alkanes as mixed C‐source have been performed. Biomass and product yields strongly depend on the C‐source of the inoculation culture. If the inoculation culture had been taken from media containing glucose as sole C‐source both glucose andn‐alkanes were utilized for cell growth in the main culture whereas only glucose was utilized if the inoculation medium contained onlyn‐alkanes.For idiophasic citrate and isocitrate production both glucose andn‐alkanes were consumed independently of the C‐source of the inoculum but that C‐source was preferentially utilized which has been the C‐source of the inoculation culture.These findings are reflected by the activities of the isocitrate lyase and the pyruvate carboxylase, respectively. InS. lipolyticaboth anaplerotic pathways are coexisting but the C‐source of the inoculation culture determines the level of the specific activities even if the ratio of the cell‐mass of the inoculum to the cell‐mass of the main culture at the end of the g

ISSN:0044-2208    

DOI:10.1002/jobm.19830230103

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

摘要:

AbstractWe have studied changes in the activity of some lytic enzymes contained in mycelium ofAspergillus nigerin cultures relative to the autolytic phase of growth. Acid phosphatase, polygalacturonidase and α‐amylase activity reached its highest level (40.7, and 8 U/sample, respectively) at the initiation of the autolytic phase of growth. 1,3‐ß‐Glucanase and ß‐N‐acetylglucosaminidase reached its highest level (3.5 and 2 U/sample, respectively) during the first days of autolysis.Alkaline phosphatase, cellulase, invertase, esterase, chitinase and proteolytic activity is also present in autolysing mycelium ofA. niger, though comparatively low. Their maximum activity coincided with the beginning of the autolytic phase of growth. In all enzymes studied here, as autolysis proceeded, enzyme activity decreased by about 90%. Only esterase activity remained nearly constant throughout the whole period of autolysis de

ISSN:0044-2208    

DOI:10.1002/jobm.19830230104

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

摘要:

AbstractUltrathin sections of early germinating endospores ofThermoactinomyces vulgariswere studied by electron microscope. Only spores aerated with an air‐CO2mixture (5% CO2) grow out, while spores aerated with air (0.03% CO2) lyse by the 25thmin of inoculation. The lysis is due to progressive, unlimited degradation of the spore integuments and a lack of cell wall formation around the spore protoplast.The requirement of CO2for outgrowth could not be replaced by oxaloacetate. CO2seems to be needed to energize the dormant cytoplasmic membrane of the spore to render it capable of initiating active transport processes and of synthesizing the germ cell wal

ISSN:0044-2208    

DOI:10.1002/jobm.19830230105

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

摘要:

AbstractAdded C1‐compounds of the intermediates methanol, formaldehyde, formate and carbon dioxide show a catalytic effect on the growth rate and cell yield of CH4‐assimilating bacteria GB 25 with serine pathway.Maximum stimulation is obtained by added amounts of about 20 mg C1‐compound/g bacteria dry matter. The influence of C1‐compounds decreases as follows: methanol>carbon dioxide>formate>forma

ISSN:0044-2208    

DOI:10.1002/jobm.19830230106

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

摘要:

AbstractDecomposition of substrates by heterotrophic microorganisms is accomplished in natural biotopes such as in soil and in waters, on or in macroorganisms but also in laboratory and industrial biotopes. The interest of man in these processes is manifold. Starting with the division of substrates into three groups of simple substrates, complex and mixed substrates with or without solid particles their qualitative and quantitative occurrence in nature and their significance in biotechnology will be discussed. In the decomposition of these substrates their utilization by pure cultures or mixed populations is to be exactly distinguished. Simple growth curves, di‐ or polyauxy, sequences of decomposition of simple substrates of a mixed substrate, population changes and successions are only some of the phenomena occurring in this process. The pathways of catabolism are subjected to manifold regulations on the three levels of stoichiometric regulation, the regulation of enzyme activity and the regulation of enzyme synthesis. In natural biotopes there is hardly a constant substrate supply over a longer period. That's why certain mechanisms of regulation are permanently acting. Thus the ‘normal’ physiological state for microorganisms is characterized by permanent transition situations – called ‘transients’. These reactions are also applied to many biotechnologica

ISSN:0044-2208    

DOI:10.1002/jobm.19830230107

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19830230110

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19830230112

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19830230101

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY