摘要:

AbstractThe pyruvate production ofE. coliML 30 in continuous cultures was investigated. In a glucose mineralsalt medium with ammonium as the limiting substrate two stable stationary states (bistability) of pyruvate concentration were obtained. The bistability was limited to dilution rates lower than 0.3 h−1and connected with a decrease of the yield coefficient (YGlc) from ≈︁0.45 to

ISSN:0044-2208    

DOI:10.1002/jobm.19770170102

出版商:Akademie‐Verlag    

年代:1977

数据来源: WILEY

摘要:

AbstractPolyploid strains ofCandida scottiishow a higher spontaneous and UV‐induced variability in accumulation of riboflavin in the medium than the original haploid strain. UV irradiation affects the formation of variants and induces those which accumulate more riboflavin than any of the most productive variants resulting from spontaneous variability. In the polyploid strains the frequency of plus‐variants increases. Therefore, polyploid strains ofC. scottiiare advantageous for selection proces

ISSN:0044-2208    

DOI:10.1002/jobm.19770170103

出版商:Akademie‐Verlag    

年代:1977

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19770170104

出版商:Akademie‐Verlag    

年代:1977

数据来源: WILEY

摘要:

AbstractA discussion of the experimental data is presented.Cells ofCandida utilisgrown in continuous culture under the influence of rather high concentrations of copper ions have been studied biochemically, morphologically, and cytologically. Although under these conditions a new steady state has been adjusted, the population is much more differentiated than under control conditions. With regard to their size, structure, and viability the cells have been arranged into 4 groups. Generally, the „copper cells”︁ in comparison with the „normal cells”︁ are more voluminous (average: 2.5 times; maximum: 4 times) and of altered structure. The appearance of dense particles („copper containing particles”︁), globules („lipid globules”︁), vacuoles, and the thickening of the cell wall, as well as the disappearance of the mitochondrial cristae have been shown as characteristic indications for damage in the cell substructure caused by the influence of copper ions. Occasionally abnormal cell shapes can be observed.A permanent influence of copper ions in the concentration used was followed also by striking disturbances of the cell metabolism. The cells take up more carbon, phosphorus, and nitrogen, whereas protein synthesis and respiratory activity decrease. Both the synthesis of lipids and polysaccharides and the phosphohydr

ISSN:0044-2208    

DOI:10.1002/jobm.19770170105

出版商:Akademie‐Verlag    

年代:1977

数据来源: WILEY

摘要:

AbstractEs wurde eine Methode zur routinemäßigen Bestimmung von Glykosiden des ϵ‐Pyrromycinons (Galirubine) in Submerskultur vonStreptomyces galilaeusausgearbeitet. Ihr liegt eine saure Hydrolyse der Galirubine, eine Extraktion des Hydrolysates mit einem nichtpolaren Lösungsmittel und eine spektrophotometrische Bestimmung des frei gewordenen ϵ‐Pyrromycinons im Extrakt

ISSN:0044-2208    

DOI:10.1002/jobm.19770170106

出版商:Akademie‐Verlag    

年代:1977

数据来源: WILEY

摘要:

AbstractEsterase in cell‐free extracts ofStreptomyces hygroscopicusJA 6599 has a temperature‐optimum of 35 °C, a pH‐optimum withp‐nitrophenylacetate as substrate at pH 7.7–8.1, with α‐naphthylacetate at pH 7–9. MICHAELIS constants in cell‐free extracts: with α‐naphthylacetateKm= = 0.71 mM, withp‐nitrophenylacetateKm= 0.21 mM. Phenylesters were better hydrolyzed than naphthylesters, phenylacetate was best hydrolyzed; β‐naphthylacetate was better hydrolyzed than α‐naphthylacetate. Among the naphthylesters the ester of propionic acid was hydrolyzed best. Caprylate, stearate, and 0,0‐diethyl‐0‐(‐p‐nitrophenyl)‐phosphate inhibit the splitting of α‐naphthylacetate. A comparison with esterases of other biological origin shows that the enzyme studied can be a carboxylesterase (E.C. 3.1.1.1.). In cultures of JA 6599 V13and JA 6599‐6 the change of esterase activity during the fermentation was determined. We found a correlation between the enzymatic activity and the a

ISSN:0044-2208    

DOI:10.1002/jobm.19770170107

出版商:Akademie‐Verlag    

年代:1977

数据来源: WILEY

摘要:

AbstractEine Methode wird beschrieben, die es gestattet, unter Verwendung der Stempelplattentechnik nach LEDERBERG eine gemischte Bakterienpopulation quantitativ in Gruppen hinsichtlich ihres Wachstumsverhaltens gegenüber verschiedenen Salzkonzentrationen aufzugliedern. Sie erfordert weniger Arbeits‐ und Materialaufwand als bisher verwendete Methoden. Die Methode wurde auf drei verschiedene natürliche Standorte angewendet: Meerwasser, Flußwasser und ein kleines flaches, stehendes, salzhaltiges Gewässer. Sie erscheint im Prinzip auch für die Aufgliederung einer Mischpopulation hinsichtlich der Beeinflussung des Wachstums durch andere Faktoren ge

ISSN:0044-2208    

DOI:10.1002/jobm.19770170108

出版商:Akademie‐Verlag    

年代:1977

数据来源: WILEY

摘要:

AbstractOut of a series of eleven related hetarylhydrazones four derivatives (ZIMET 124/73, ZIMET 38/74, ZIMET 44/69, and IMET 98/69), after subcutaneous administration (0.5 mmole/kg per injection), were found to possess significant antiviral activity against rapidly progressing and highly lethal Mengo virus encephalitis in mice. After peroral administration (2 mmole/kg per injection) only with IMET 98/69 significant protection was achieved. Drug treatments were given twice daily for 5 days. The effectiveness of compounds was evidenced by significant „rates of protection”︁. Possible relationships between the antiviral activity and the structure of compounds are disc

ISSN:0044-2208    

DOI:10.1002/jobm.19770170109

出版商:Akademie‐Verlag    

年代:1977

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19770170110

出版商:Akademie‐Verlag    

年代:1977

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19770170111

出版商:Akademie‐Verlag    

年代:1977

数据来源: WILEY