摘要:

In a retrospective study, we examined 34 premalignant lesions of the oral cavity by flow cytometer analysis on formalin‐fixed, paraffin‐embedded tissue submitted to the University of Tennessee. Memphis, Oral Pathology Laboratory. Three categories of oral epithelial dysplasia were represented (eight mild, seven moderate, nine severe), as well as live epithelial hyperplasias without dysplasia and five fibrous nodules as controls. The DNA index and total proliferative index of each ease were calculated. The objective of the study was to determine the amount of epithelial dysplasia necessary in oral lesions before DNA aneuploidy or high proliferative index is detectable and thus determine if How cytometric analysis can be a diagnostic adjunct for oral premalignant lesions. The results showed that some cases in both the control and dysplastic categories exhibited a high total proliferative index (control = 1, no dysplasia = 1, mild dysplasia = 3, moderate dysplasia = 2, severe dysplasia = 2), whereas only the dysplastic lesions had an abnormal DNA index [8 of 24 (33%)]. The results indicate that flow cytometric analysis may have some limited potential as a diagnostic adjunct in oral premalignant lesi

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1992.tb00959.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The keratin expression pattern in oral stratified epithelium is related to the cellular differentiation level. The normal pattern shows the keratin pair K5 and K14 in the stratum basale whereas K1 and K10, or K4 and K13, are the two pairs associated with differentiating suprabasal cells. Monoclonal and polyclonal antibodies to individual keratins (K10, K13 and K14) were used in a two‐color imnuinofluorescence staining method to study their coexpression in single cells. Altered keratin expression in premalignant and malignant lesions indicated abnormal differentiation. Monospecific keratin antibodies were suggested to be useful for evaluation of epithelial differentiation changes in oral dysplasias and oral squamous cell carcinoma

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1992.tb00960.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The oral cavity, and particularly the gingival mucosa, is continuously exposed to numerous food and bacterial plaque antigens, though evident immunologic reactions are uncommon. It is therefore possible that the mucosal associated lymphoid tissue (MALT) of this region is preferentially biased towards unresponsiveness, rather than immune cell activation. The distribution and phenotype of immune cells in normal human gingiva were examined. Their distribution varied, and high and low cellularity areas could be distinguished in the same specimen. The number of CD3 positive (CD3+) T lymphocytes was more than thrice higher in a high cellularity area. In both types of area, intraepithelial T lymphocytes were not activated. Moreover, they showed chromatin condensation and cell shrinkage characteristic of apoptosis. In the stroma of high cellularity areas, foci of cell activation and numerous B cells were present, suggesting a localized active immune response. The vast majority of intraepithelial and stromal T lymphocytes expressed the “memory” CD45RO+ phenotype. The absence of an immune response within the epithelium and the localized response in the stroma (probably due to the binding of memory T cells to antigens in a low affinity, cross‐reactive fashion) may be a part of a protective mechanism against indiscriminate stimulation by a multitude of external ant

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1992.tb00961.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Cellular expression of keratins (intermediate filaments) can be demonstrated by immunocytochemistry using unfixed tissue. However, for practical reasons, provision of fresh tissue to the laboratory is often difficult. Recently a fresh tissue transport medium (Patent applied for) has been developed which allows keratin immunocytochemistry to be performed up to 4 days after biopsy. In this study oral mucosal biopsies from 10 patients were hemisected, half placed in the new transport medium at 4 C and the other half immediately frozen in liquid nitrogen. After 4 days frozen sections of both halves of the biopsies were stained by immunocytochemistry using various antikeratin antibodies. The morphology and staining characteristics of the two halves of the biopsies were then assessed. No significant difference could be found in either morphology or preservation of keratin expression in specimens stored in transport medium, as compared to those in liquid nitrogen. This new transport medium may offer considerable advantage for the provision of a histologic and immunocytochemical diagnostic service.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1992.tb00962.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Much evidence suggests that recurrent oral aphthous ulceration (RAU) is an immunologically mediated disease. Tumour necrosis factor has multiple biologic properties, some of which may be relevant to the pathogenesis of RAU. This study has assessed its production by peripheral blood leukocytes from aphthous patients in active and remission phases of disease and from patients with non‐aphthous ulceration (diseased controls). Each ulcer patient was studied in parallel with a matched healthy control volunteer. A bioassay against the standard mouse fibrosarcoma line, L929, was used to assess the levels of tumour necrosis factor‐a (TNF). Significantly greater amounts of TNF were released from unstimulated monocyle‐enriched and monocyte‐depleted leukocyte fractions in active RAU compared with those from healthy control donors, suggesting that this cytokine may be associated w

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1992.tb00963.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Serum levels of immunoglobulin G (IgG) subclasses were examined in 71 adult patients with minor recurrent aphthous stomatitis (MiRAS). Seven patients (10%) had altered IgG subclasses: six had marginally raised IgG1 levels and one had a low level of IgG3. It is concluded that abnormalities of IgG subclass levels do not underlie MiRAS.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1992.tb00964.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Langerhans cells (LC) serve as antigen presenting cells and provide immune surveillance within epithelia. Since depression of LC number and/or function may allow tolerance to antigens, we evaluated LC in median rhomboid glossitis (MRG), a condition linked to persistent candidal infection of lingual mucosa. Material included a total of 36 cases of MRG (7 of which did not show PAS+fungi) and 6 controls. LC were identified by their expression of S‐100 and HLA‐DR antigens and quantified using image analysis. Equal numbers of LC were identified using S‐100+ and HLA‐DR+ markers. The density of LC (cells/mm of basement membrane, mean±SD) in both PAS+ MRG (2.6±1.3) and PAS‐MRG (3.0±1.7) was markedly depressed compared with controls (17.2±6.4), (P<0.001). These findings indicate that the LC network is perturbed in MRG, and are consistent with the view that of localized defect in immune surveillance may contribute to persistent fungal infection of th

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1992.tb00965.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Taste detection and recognition thresholds were monitored in 10 patients for up to 1 yr after allogeneic bone marrow transplantation (BMT). As well as a control group of 12 healthy volunteers, taste acuity was tested in 10 patients, who had undergone BMT 2–5 yr previously. Immediately after transplantation, there was significant hypogeusia of all four taste modalities compared to registrations one week before the a plastic phase and also compared to the healthy control group. Although some normalization of taste thresholds was registered 3–6 months after transplantation, most subjects still experienced dysgeusia. Of the four taste modalities, the most frequently recorded change was a raised threshold for salt. In about 80% of the patients taste acuity had recovered to the control values one year after transplantation. The group tested 2–5 yr after BMT had normal values for taste a

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1992.tb00966.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Opercula of permanent first and second molars delayed in eruption were investigated histologically to detect possible causes of eruption failure. The material comprised operation specimens from exposure of 74 non‐erupted molars in 63 young individuals (34 boys. 29 girls). Eighteen of the 74 specimens (or 24.3%) were diagnosed as “classical” odontogenic tumors belonging to the following entities: ameloblastic fibroma (seven), ameloblastic flbrodentinoma (six), ameloblastic fibro‐odontoma (four) and complex odontoma (one). Twenty‐two specimens (or 29.7%) showed a hitherto unrecognized odontogenic lesion of hamartomatous character, termedodontogenic giant cell fibromatosis IGCF).Thus, 54.1% of the specimens could be diagnosed as odontogenic tumors or humartomas. Histomorphologic changes could not be detected in the remaining 34 specimens (45.9%). Odontogenic tumors were associated with uneruptedfirstmolars much more frequently than withsecondmolars (ratio 8:1). The OGCF had a strong association with uneruptedmandibularmolars. Further, opercula ofmandibular firstmolars frequently revealed odontogenic lesions whereas tissue overlying the crown of uneruptedmaxillary secondmolars often was reported as normal

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1992.tb00967.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Cysts of this site are quite common in mature ewes in New Zealand and the British Isles, although their true prevalence is not yet known. In most examples, an unerupted incisor is embedded in their wall but some do not contain a tooth. The cysts are lined by parakeratinized stratified squamous epithelium and generally exhibit a flat interface between the epithelium and the connective tissue. They may become secondarily infected, in which case they exhibit chronic inflammation. In some cases, foci of cementum are attached to the epithelial lining. When a tooth is involved, the cysts arise from various locations along its crown, which in sheep is normally covered by a thin layer of cementum. Some of these unerupted teeth exhibit irregular hypercementosis and/or external resorption which is repaired by cementum. In some examples there is a fistula between the cyst and the oral cavity, either through a deep periodontal pocket or from the incisal edge of the unerupted tooth piercing the overlying mucosa.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1992.tb00968.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY