摘要:

To assess the potential role of heat shock protein (HSP) in the pathogenesis of oral lichen planus (OLP), sections of OLP, normal oral mucosa, non‐specific oral ulceration (NSOU) and dysplastic OLP were assessed for HSP expression using avidin‐biotin complex immunohistochemistry with an anti‐HSP 70 polyclonal antibody. There were statistically significant differences in both the vertical and horizontal staining distribution when other groups were compared with the OLP group (p<0.01). Using microdensitometry, the mean staining intensity in OLP, dysplastic OLP and NSOU was elevated in comparison with normal oral mucosa (p<0.001). In a standard tritiated thymidine uptake assay, lymphocytes extracted from nine OLP lesions demonstrated significant proliferation when stimulated with purified protein derivative (PPD), of which HSP is a major constituent, with stimulation indices ranging from 2 to 132. These results are consistent with the hypothesis that, in OLP patients, diverse exogenous agenst may cause upregulated expression of HSP by oral mucosal keratinocytes. A reaction of cytotoxic T lymphocytes to these activated keratinocytes may then result in the tissue destruction which is characteristic of OLP le

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01121.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Erythema multiforme may be recurrent and the oral cavity is often affected. A series of 82 patients with unequivocal recurrent cutaneous erythema multiforme were examined to determine the incidence and nature of oral lesions. Seventy per cent of patients had oral lesions, comprising multiple, large, shallow, extremely painful and debilitating ulcers, which affected the entire oral mucosa in over 20%. The buccal mucosa and tongue were the most frequently affected sites in the remainder and the lips were affected in 13% of patients. Lesions generally lasted for 1–3 weeks. In over 60% of cases these attacks followed an episode of herpes simplex virus infection during the preceding fortnight. Recurrent attacks showed a different site distribution from the initial attacks, with a greater proportion having genital as well as skin and oral mucosal involvement. Detailed case histories of five patients are presented to illustrate the role of azathioprine in treating oral lesions and to document a familial case of recurrent erythema multiforme, with severe mucosal involvemen

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01122.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The possible involvement ofCytomegalovirus(CMV) and Helicobacter pylori (HP) in oral mucosal ulcers is suggested by their role in the development of ulceration at other mucosal sites of the gastrointestinal tract. A series of 29 incisional biopsies from 29 consecutive and apparently immunocompetent patients attending the clinic for oral ulceration were examined by routine histopathology as well as byin situhybridisation (ISH) with biotinylated CMV and HP DNA probes. In 14/29 biopsies, Giemsa staining disclosed spiral bacteria. Six (20.7%) of these 14 Giemsa‐positive samples showed HP DNA on ISH and 3 ulcers (10.3%) contained CMV DNA. In none of the specimens were CMV and HP detected simultaneously. Two of the ulcers containing CMV DNA were found on the labial mucosa and one on the posterior palatal mucosa, whereas all HP DNA‐positive ulcers were located on the buccal mucosa. The results indicate that CMV and HP DNA can be found in separate oral mucosal ulcers in apparently immunocompetent adu

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01123.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Immunohistochemically detectable levels of p53 may be seen early in the malignant transformation of some neoplasms. To determine if p53 is immunocytochemically detectable, and therefore presumptively abnormal, in oral dysplasias and in situ carcinomas, and to explore the natural history of p53 protein expression in these lesions, sequential biopsies from patients with lesions occurring in the same anatomic site were examined. Formalin‐fixed, paraffin‐embedded sections from 19 patients were evaluated immunohistochemically for p53 protein using antibody clones Pab1801 and BP53‐12. With two exceptions, comparable results were observed with these antibodies. p53 protein was detected immunocytochemically in 6 of 13 patients with dysplasias; 3 of these progressed to p53‐positive invasive carcinoma, one advanced to a more severe grade of p53‐positive dysplasia, one developed into a p53‐negative verrucous carcinoma, and one represented a p53‐positive dysplasia developing five years after treatment of a p53‐positive carcinoma. The p53‐positive dysplasias, which were found in all subtypes (mild, moderate, severe), preceded histologic malignant change by months to years. p53 detection was evident in 4 of 6 patients with in situ lesions. Sequential biopsies of three of these lesions showed no change in lesion histology or p53 staining, and one lesion advanced to a p53‐positive carcinoma. It is concluded that p53 protein may be detected early in the development of a subset of p53‐positive oral squamous cell carcinomas. This phenomenon may be seen in dysplasias and in situ lesions, and it may have pr

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01124.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

This study characterised the nature of the local cellular immune responses associated with an inbred animal model of oral carcinogenesis. Inbred F344 rats developed moderately‐ to well‐differentiated primary oral squamous cell carcinomas (SCC) after treatment with the carcinogen 4‐nitroquinoline N‐oxide (4‐NQO)in vivofor 5–6 months. The inflammatory cell infiltrate associated with the primary tumours was predominantly of the macrophage lineage (CD45+, Ia+) and contained smaller numbers of CD8+cells (NK cells, cytotoxic/suppressor T cells), CD5+cells (T cells) and CD25+cells (activated cells; T and NK cells). Keratinocyte cell lines were established from three lingual and one palatal SCC. By contrast to normal keratinocytes, tumour‐derived cell lines were immortal and independent of 3T3 fibroblast support. All of the tumour‐derived cell lines were tumorigenic in athymic (nu/nu) mice and showed contrasting latent periods of tumour development and histological differentiation; normal keratinocyte grafts were non‐tumorigenic in athymic mice. Three of four malignant cell lines formed well‐differentiated tumours in syngeneic F344 rats; the tumours regressed after 10–14 days. Regressing grafts contained significantly larger numbers of NK cells (CD5‐, CD8+) in the inflammatory cell infiltrate compared with that associated with primary tumours (p<0.04). One malignant cell line and normal keratinocytes were non‐tumorigenic in syngeneic hosts. The results demonstrate phenotypic variation in the cell‐mediated immune responses associated with the actively growing primary SCC and the regressing tumours in syngeneic hosts and suggest that NK cells, possibly activated by local T cell responses, are important for tum

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01125.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

OralCandida albicansisolates from HIV‐infected individuals in Hong Kong, Australia, Germany and England were characterised using a biotyping system based on enzyme profiles, carbohydrate assimilation patterns and boric acid resistance of the yeasts. A total of 44 biotypes were found amongst the 117 oralC. albicansisolates examined. The major biotype AIR accounted for 17.9% of all isolates while the second commonest biotype was A1S (11.1% of isolates). Whereas these two biotypes were isolated from all the regions studied, there were a number of other biotypes unique to individual countries. The data indicate that there are many different sub‐strains of oralC. albicansin HIV‐infected patients, some of which are globally prevalent. However, further work is required to ascertain the diversity of oralC. albicansbiotypes, if any, in health and di

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01126.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

This study was undertaken to examine the relationship between malignant tumor tissue and nerve fibers using a transplantation model and nerve fiber stains. Most of the neural tissue, as well as the other host tissues, showed severe degenerative changes as the transplanted tumor grew. Furthermore, the degenerative changes were chiefly confined to the area anterior to the tumor. These changes were probably provoked chiefly by the compressive effects of the rapidly enlarging transplant. The results could possibly explain clinical symptoms such as paralysis or disturbances in the movement of the tongue observed occasionally in some patients with lingual carcinomas.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01127.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

This article presents six cases of oral psoriasis originally diagnosed by means of the histological changes found on biopsies of the oral lesions. Three of the patients presented with oral and skin psoriasis. Of the remaining three, two presented with oral manifestations alone, although their follow‐up was short, whilst the third showed delayed dermatological changes. Two of the cases were also complicated by psoriatic arthritis, one of them to the temporomandibular join

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01128.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Recurrent oro‐genital ulceration is a common condition of unknown aetiology. This paper describes a patient who had severe recurrent oro‐genital ulceration which was unresponsive to conventional therapy. The patient was taking non‐steroidal anti‐inflammatory drugs (NSAIDs) prescribed for osteoarthritis. When she stopped this medication, she had no further genital ulceration and the pattern of her oral ulceration was dramatically improved. There have been no previously reported cases of recurrent oro‐genital ulceration associated wi

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01129.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY