摘要:

A case is described of Osteosarcoma arising in a pre‐existing fibrous dysplasia fifty years after radiotherapy for facial hirsutism. The patient was treated by surgical excision and chemotherapy and is alive and free of disease five years late

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00555.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

An immunohistochemical survey of the distribution of keratin was studied in chemically induced carcinomas of the submandibular glands of mice. Initial signs of premalignant changes were degranulation of granular convoluted tubule cells and deposition of keratin protein in small limited areas of the degranulated cells. There was a gradual increase in the area showing keratin staining in altered tubule cells. Duct‐like and cystic structures stained intensely for keratin, as did squamous metaplastic epithelial cells. Induced carcinomas were variably keratinized. Basal layers of cells of squamous‐cell carcinomas displayed weak keratin staining, and spinous tumor cells and parakeratotic tumor cells showed somewhat increased levels of keratin staining. Some desquamated keratotic tumor cells stained intensely for keratin. Just as the localization of epidermal and nerve growth factors and lectin‐binding histochemistry have been used in studying tumorigenesis in the mouse submandibular gland, immunohistochemically detected keratin proved to be a useful marker of tumor cells of ductal segment o

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00556.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The periodontal membrane of European hamster maxillary incisors showed histologic changes consisting of an irregularly orientated, less vascularized connective tissue which included abundant cementicle‐like structures. The changes were multilocal and bilateral, growing towards the alveolar bone (Grade 1), causing atrophy of the alveolar bone due to compression (Grade 2) and reaching the periosteal membranes (Grade 3). In a few animals, the lesions were seen after one year. They were seen in 80% of hamsters more than 2 years old. Carcinogenic exposure did not alter the latency and incidence of the alterations. The lesions were thought to be a spontaneously occurring disease representing a nodular cemento‐fibrous dysplasia of the periodontium. As, morphologically, no exact attribution to other odontogenic or mesenchymal growth could be made, the entity of this periodontal lesion was considered. Although the changes observed in the periodontal membrane of the maxillary incisors of the European hamster were not exactly comparable to odontogenic cemental lesions in man, they may serve as a model to further clarify the nature of fibro‐osseous le

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00557.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Immunohistochemical detection of keratin proteins in duct‐ligated submandibular glands (SMG) was carried out in mice and rats with or without testosterone administration. Keratin staining in normal salivary glands was limited to the striated duct (SD) and excretory duct (ED) cells and was usually lacking in granular convoluted tubule (GCT) cells. Following duct‐ligation, the epithelia of intercalated ducts (ICD), degranulated tubules, duct‐like structures, and dilated striated and excretory ducts showed positive keratin staining, usually in their luminal aspects. The concentration of keratin was proportional to the degree of degranulation of the GCT cells. The duct‐ligated SMGs in animals with testosterone treatment showed a comparatively higher number of granules located in the GCT cells, and degranulation was slight. Keratin staining in hormone‐treated duct‐ligated glands also occurred in ductal segments to a slight degree. Keratin was also detected in degranulated tubules, and its concentration was increased in duct‐like structures, whereas staining for EGF and NGF was decreased in degranulated tubules and lacking in duct‐

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00558.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The purpose of this study was to investigate histologically the effect of wounding on the hamster tongue after pretreatment with DMBA. The animals in which the tips of the tongues were pretreated with DMBA for 8 weeks, subsequently excised, and had no treatment or received applications of acetone, showed epithelial dysplasias. The animals which had the same pretreatment, excision, and received additional post‐excision applications of DMBA for 9–13 days, developed squamous cell carcinomas. However, the animals which were pretreated with DMBA for 8 weeks but had no excision, did not show any pathologic changes, even though they received additional applications of DMBA for 9–13 days. The animals which received no pretreatment with DMBA for 8 weeks hut had an excision, showed normal wound healing, even though they had a post‐treatment with DMBA for 9‐13 days. The results of the present study indicated that excisional wounding acted as a promotional stimulus in inducing the appearance of epithelial dysplasias or carcinomas of tongues initiated with the carcin

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00559.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Electron microscopy of the intact surface zone of white spot and brown spot carious lesions showed that in general their ultrastructure was similar. Their outermost crystalline surface consisted of small crystals similar to those in healthy enamel, crystals with central core dissolution, and rounded crystals. Below this, surface demineralization of enamel was observed as the enlargement of micropores, the central core dissolution of crystals, the formation of channels and the enlargement of spaces at prism boundaries. Remineralization of enamel was observed as the partial occlusion of voids, the rounding and enlargement of crystals, and some new needle‐shaped crystals. Some other features indicated combined demineralization and remineralization. The occlusion of spaces at prism boundaries was a more common feature in brown spot lesions, whereas the pockets of rounded crystals were more common in white spot lesions. A relatively uniform distribution of needle‐shaped crystals throughout the intact surface zone was a feature of some brown spot lesions o

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00560.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

We have examined the keratin proteins in normal human oral mucosa from 6 different regions including hard palate, buccal mucosa, tongue, gingiva and floor of the mouth. Urea‐dithiothreitol extracts of EDTA separated epithelia were analysed by SDS‐PAGE and immunoblotting. Eight samples from each region were investigated and showed very little individual variation in the keratin profile on Coomasic Blue‐stained gels. The keratinizing hard palate and gingiva expressed identical patterns and resembled the pattern of epidermis from the flank region. The normally non‐keratinizing buccal mucosa and the mucosa of the floor of the mouth expressed polypeptides distinctly different from those of the keratinizing epithelia and lacked the high molecular weight keratins. The dorsal surface of the tongue and the commissure region showed a pattern intermediate between keratinizing and non‐keratinizing epithelia. The greater sensitivity of the immunoblotting technique revealed that the non‐keratinizing epithelia synthesized one of the high molecular polypeptides and that the tongue produced all the bands found in keratinizing epithelia, but in very small quantities. There are, thus, distinct differences in the keratin expression of oral epithelia which are related to the pattern of keratinization assessed his

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00561.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

While there is considerable evidence that skin carcinogenesis proceeds as a step‐wise series of changes, little evidence is available to indicate that a similar mechanism applies to oral mucosal carcinogenesis. In the current study, a mouse model of mucosal carcinogenesis was used to examine the effects of repeated applications of the phorbol ester phorbol‐12,13‐didecanoate (FDD) following various periods of treatment with the carcinogen 4‐nitroquinoline‐1‐oxide (4NQO). All animals were histologically examined at 50 weeks. Oral epithelial dysplasia was seen in animals treated with 4NQO for as little as 2 weeks, and oral squamous cell carcinomas developed in all animals treated with 4NQO for 12 weeks. In those mice treated with PDD as well, carcinomas developed in mice receiving as little as 2 weeks treatment with 4NQO, and 100% of mice treated with 4NQO for 12 weeks, followed by PDD treatment, developed carcinomas. The results indicate that irreversible changes in the oral mucosa of mice occur relatively early during treatment with 4NQO, and the development of carcinomas can be enhanced with subsequent PDD treatment, giving an indication of staged carcinogenesis in this m

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00562.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Ten oral verruciform xanthomas were studied using an immunoperoxidase stain for S‐100 protein. All cases exhibited positively stained dendritic cells among the mononuclear inflammatory cell infiltrate at the base of the lesions and to a lesser extent among the “foam cells”. The foam cells were, however, negative for S‐100 staining. We suggest that, based on these findings, verruciform xanthomas belong to a new category of “non‐X histiocytoses” in which the presence of Langerhans cells suggests an immunologic

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00563.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

AbstractThis study examines chemically and histologically the relative abilities of inositiol monophosphate (IP1), inorganic phosphate (Pi), ethane‐1‐hydroxy‐1, 1‐diphosphonate (EHDP) and dichloromethylene disphosphonate (Cl2MDP) to inhibit parathyroid hormone (PTH) ‐ induced resorption of fetal rat long bones in organ culture. Pregnant rats injected with45Ca on the 18th day of gestation were killed the next day and their fetuses removed. Half of each pair of dissected long bones was incubated in a chemically defined control medium while the contralateral half was incubated in medium containing PTH or PTH plus the compound to be tested.45Ca released into the medium was indicative of the amount of bone resorption. Bones were then processed histologically and examined microscopically. All compounds inhibited resorption to some extent with IP1 and Pi being less effective than EHDP or Cl2MDP at comparable phosphate concentrations. However, the disphosphonates damaged osteoclasts whereas IP1 and Pi did not. This suggests that IP1 may inhibit resorption by a different mechanism perhaps related only to prevention of crystal di

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00564.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY