Culture and Cryopreservation of Chondrocytes from Human Cartilage: Relevance for Cartilage Allografting in Otolaryngology
作者:
J. Bujía,
P. Pitzke,
E. Wilmes,
C. Hammer,
期刊:
ORL
(Karger Available online 1992)
卷期:
Volume 54,
issue 2
页码: 80-84
ISSN:0301-1569
年代: 1992
DOI:10.1159/000276267
出版商: S. Karger AG
关键词: Reconstructive surgery;Cartilage transplantation;Cell-tissue culture;Cryopreservation
数据来源: Karger
摘要:
One of the reasons for failure of cartilage allografts is the impaired condition of the transplant during storage. In this paper we describe methods for the isolation and culture of viable chondrocytes obtained from nasal septum cartilage. Furthermore, we evaluate the possibility of growing such specific chondrocytes under culture conditions and storing them in a frozen state. Age dependent differences were observed in the growth rate of the cultured cells. Our results confirm that chondrocytes survive freezing and remain able to proliferate. Knowledge gained from this study may be applied to the culture and freezing of viable intact cartilage for use in reconstructive surgery in otolaryngology.
点击下载:
PDF
(2073KB)
返 回