Improved High Performance Liquid Chromatographic Method for the Determination of Tris(Hydroxy-Methyl)Aminomethane (THAM) in Human Plasma, Erythrocytes, and Whole Blood
作者:
R.N. Nivarthi,
M. Kanchuger,
L. Yarmush,
H. Turndorf,
M. Bansinath,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1998)
卷期:
Volume 21,
issue 11
页码: 1701-1715
ISSN:1082-6076
年代: 1998
DOI:10.1080/10826079808001253
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A sensitive high performance liquid chromatographic (HPLC) assay was developed to quantitate the organic amine proton-acceptor, tris(hydroxymethyl)aminomethane (THAM) in human plasma, erythrocytes, and whole blood. An aliquot of the sample was heat evaporated (200°C for 2 h), derivatized, extracted, and then injected onto a symmetry C8column. The UV absorbance of the effluent was monitored at 237 nm. 2-Amino-2-methyl-1,3-propanediol was found to be an appropriate internal standard. The method has been applied to quantitate the samples from patients undergoing orthotopic liver transplantation. The assay has also been employed to assess thein vitrotime of THAM-uptake into the erythrocytes from blood samples incubated at 37°C.
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