Adherence of Viable and Nonviable Bacteria to Soft Contact Lenses
作者:
Thomas John,
Miguel Refojo,
Laila Hanninen,
Fee Leong,
Antonio Medina,
Kenneth Kenyon,
期刊:
Cornea
(OVID Available online 1989)
卷期:
Volume 8,
issue 1
页码: 21-33
ISSN:0277-3740
年代: 1989
出版商: OVID
关键词: Soft;contact;lens;Hydrogels;Pseudomonas;aeruginosa;Viable;bacteria;Nonviable;bacteria;Bacterial;adherence
数据来源: OVID
摘要:
Bacterial viability in bacterial adherence to new soft contact lenses (SCLs) was studied by exposing polymacon and lidofilcon A lenses toPseudomonas aeruginosa:L, live, untreated bacteria; H, bacteria heated for 1 h at 100°C; and P, bacteria treated with 3% hydrogen peroxide for 1 h. Cultures of groups L, H, and P showed heavy, no, and minimal bacterial growth, respectively. Scanning electron microscopy (SEM) showed significantly less bacteria on new SCLs in groups H and P. Transmission electron microscopy (TEM) showed that bacterial shape was mostly preserved in all groups, with marked intracel-lular and cell membrane changes and breaks (groups H and P), margination of intracellular electron-dense material (group H), and central and peripheral vacuolation (group P). Adherence to new SCLs appears to be an active process, as killed or altered bacteria adhere less.
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