Detection of Quinidine‐Specific Antibodies with Platelet125J‐Labeled Staphylococcal Protein A Test
作者:
R. Kekomäki,
A. Rajamäki,
G. Myllylä,
期刊:
Vox Sanguinis
(WILEY Available online 1980)
卷期:
Volume 38,
issue 1
页码: 12-18
ISSN:0042-9007
年代: 1980
DOI:10.1111/j.1423-0410.1980.tb02324.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
Abstract.Remarkably high titers (80–160) of quinidine‐specific antibodies were detected in the serum of 5 patients with suspected quinidine purpura, when126I‐labeled staphylococcal protein A was used as the marker of IgG bound onto platelets. During a follow‐up of 3 of the patients, the antibody titers declined.The antibodies could be detected even when the final drug concentration was reduced from 0.3 to 0.003 mmol/I (i.e. therapeutic levels). The reaction between serum and platelets was independent of the type of quinidine salt used. No cross‐reactivity occurred with quinine.Heat inactivation of the serum did not decrease the quantity of specific antibodies detected on the platelets. The sedimentation behavior of antiplatelet activity in density gradient centri‐fugation resembled that of 7 S antibodies. When sedimentation was, however, effected on the drug‐serum mixture, the reactivity of
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