Flow injection chemiluminescence study of acridinium ester stability and kinetics of decomposition
作者:
Janet S. Littig,
Timothy A. Nieman,
期刊:
Journal of Bioluminescence and Chemiluminescence
(WILEY Available online 1993)
卷期:
Volume 8,
issue 1
页码: 25-31
ISSN:0884-3996
年代: 1993
DOI:10.1002/bio.1170080106
出版商: John Wiley&Sons, Ltd.
关键词: Acridinium ester;chemiluminescence;flow injection;stability;decomposition;kinetics
数据来源: WILEY
摘要:
AbstractDecomposition of phenyl acridinium‐9‐carboxylate is monitored using electrogenerated chemiluminescence in a flow system. The formation of the pseudobase from the acridinium ester [AE] is described by rate =k′1[AE] +k″1[AE][OH−]0.5, wherek′1= 0.020 ± 0.006 s−1andk″1= 2.1 ± 0.8 (L/mol)−0.5s−1. Irreversible decomposition of the pseudobase is described by rate =k′2[AE][OH−], wherek′2= 20.1 ± 3.8 (L/mol s). These kinetic equations, plus measurement of variation in emission intensity for constant acridinium ester concentration, are used to predict the resulting emission intensity v. pH behaviour given various contact times (in the 0.25 to 25 s range) for the acridinium ester to be in an alkaline solution prior to initiation of th
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