The athymic nude rat. IV. Immunocytochemical study to detect T‐cells, and immunological and histopathological reactions against Trichinella spiralis
作者:
J.G. VOS,
E.J. RUITENBERG,
NETTY VAN BASTEN,
JANNIE BUYS,
ANNEKE ELGERSMA,
W. KRUIZINGA,
期刊:
Parasite Immunology
(WILEY Available online 1983)
卷期:
Volume 5,
issue 2
页码: 195-215
ISSN:0141-9838
年代: 1983
DOI:10.1111/j.1365-3024.1983.tb00737.x
出版商: Blackwell Publishing Ltd
关键词: athymic nude rat;Trichinella spiralis;immunity;worm kinetics;connective tissue mast cell;intestinal mast cell;globule leucocyte
数据来源: WILEY
摘要:
SummaryThe anti‐parasite response was investigated after oral infection of athymic nude (rnu/rnu) rats and heterozygous (+/rnu) littermates with 1000 muscle larvae of Trichinella spiralis. No IgM, IgG and IgE antibodies were detected in serum of rnu/rnu rats. Expulsion of adult worms from the small intestine was prolonged (worms were nearly all expelled at days 14 and 91 in +/rnu and rnu/rnu rats respectively). The yield of muscle larvae in the carcasses of nude rats at day 91 was 33 times higher than in +/rnu rats. In contrast to the strong inflammatory reaction in the parasitized tongue of +/rnu rats, no infiltration was observed in rnu/rnu rats. Using an immunoperoxide method with monoclonal anti‐rat T‐cell antibody, no T cells were identified in spleen, mesenteric lymph node and Peyer's patches. These data support earlier studies that the nude rat lacks functional T cells. As the counts of connective tissue mast cells (CTMC), intestinal mast cells (IMC) and globule leucocytes (GL) in small intestine of uninfected rnu/rnu rats were equal or higher than in +/rnu rats, it is concluded that the origin of these cells is thymus‐independent. In contrast to +/rnu rats, infection of rnu/rnu rats induced no increase of CTMC, IMC or GL. Thus, these cells depend on T cells to undergo proliferation. Finally, results of this study were inconclusive whether IMC are precursors for GL, or that they represent independent cell popu
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