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ISOELECTRIC FOCUSING IN POLYACRYLAMIDE GELS

 

作者: Lorraine H Percival,   GG Dunckley,   HD Purves,  

 

期刊: Australian Journal of Experimental Biology and Medical Science  （WILEY Available online 1970）
卷期: Volume 48, issue 2  

页码: 171-178

 

ISSN:0004-945X

 

年代: 1970

 

DOI:10.1038/icb.1970.16

 

出版商: Nature Publishing Group

 

数据来源: WILEY

 

摘要:

SummaryAn improved technique for isoelcctric focusing of proteins in polyacrylamide gels is described.In studying insulin with the procedure, mercuric chloride in trichloro‐acetic acid was used to fix the protein in the gel.One major and five minor components were demonstrated in ox and pig insulin.

 

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