Immunohistochemical Detection of Activin A in Osteoclasts
作者:
Takayuki Hosoi,
Satoshi Inoue,
Shinjiro Hoshino,
Yasuyoshi Ouchi,
Hajime Orimo,
期刊:
Gerontology
(Karger Available online 1996)
卷期:
Volume 42,
issue 1
页码: 20-24
ISSN:0304-324X
年代: 1996
DOI:10.1159/000213821
出版商: S. Karger AG
关键词: Activin A;Osteoclast;Bone-resorbing lacunae
数据来源: Karger
摘要:
Production of a member of transforming growth factor-β (TGF-β) superfamily, activin A, was examined in the bone tissue by using reverse transcriptase polymerase reaction. As a result, specific bands were detected showing the presence of activin A mRNA in the bone tissues. In order to localize the production site of activin A in the bone tissues, we tried to immunolocalize activin A in fetal mouse calvaria cultured in a medium containing fetal calf serum, 1α-25(OH)2 vitamin D3 and parathyroid hormone. In these cultured calvaria, bone tissues including bone-resorbing osteoclasts in vitro were observed. Positive staining demonstrating the presence of activin A resided inside of the multinucleated cells in the bone-resorbing lacunae, suggesting the production of activin A in osteoclasts. Activin A was also localized immunohistochemically in the osteoclast-like multinucleated cells developed in vitro. These results suggest that osteoclasts produce activin in the bone tissues and that activin may play some roles by auto-crine and/or paracrine manner in bone metabolis
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