首页   按字顺浏览 期刊浏览 卷期浏览 In VitroCulture and Somatic Embryogenesis of White Clover (Trifolium Repens L.)
In VitroCulture and Somatic Embryogenesis of White Clover (Trifolium Repens L.)

 

作者:

 

期刊: Biotechnology & Biotechnological Equipment  (Taylor Available online 1995)
卷期: Volume 9, issue 1  

页码: 10-13

 

ISSN:1310-2818

 

年代: 1995

 

DOI:10.1080/13102818.1995.10818815

 

出版商: Taylor & Francis

 

数据来源: Taylor

 

摘要:

A comparative examination in vitro of the morphogenetic characteristics of two cultivars and one candidate-cultivar of white clover (Trifolium repens L.) was carried out. Callus cultures were initiated from hypocotyl and cotyledon's tissues on five nutrient culture media. Embryo-like structures were generated in callus cultures only induced on MS nutrient medium containing 2 mg/l 2,4-D, 0.2 mg/l kinetin and 1 mg/l adenine. This medium turned out expediently balanced as for callus tissue initiation as well as for somatic embryoide induction. The complete development of differentiated structures to a plant was effectuated on MS culture medium with addition of 0.2 mg/l 2,4-D and 1 mg/l kinetin. In some of the obtained regenerants morphological deviations were established.

 

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