Lytic Activities, Protein Profiles and Morphologic Characteristics of New Bacteriophages Isolated from Canine and HumanStaphylococcus aureusStrains
作者:
A. A. Adesiyun,
A. Viebahn,
H. G. Sahl,
W. Lenz,
K. P. Schaal,
期刊:
Journal of Veterinary Medicine, Series B
(WILEY Available online 1992)
卷期:
Volume 39,
issue 1‐10
页码: 39-47
ISSN:0931-1793
年代: 1992
DOI:10.1111/j.1439-0450.1992.tb01135.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
SummaryThe lytic activity, protein profile and morphology of five newly isolated phages from canineStaphylococcus aureusstrains and one from a humanS. aureusstrain were compared with those of selected phages in the international phage sets (IPS). Five canine phages lysed 57 (76.0 %) of 75 canine isolates ofStaphylococcus aureusfrom Nigeria at routine test dilution (RTD) while 34 (IPS) phages typed only 31 (41.3 %) strains at RTD or/and 100‐RTD. The new human phage lysed 11 (14.7%) of 75 strains isolated from human diarrhoea. The new phages were readily propagated, specific in activity and stable during storage at 4°C.Prominent proteins detected by SDS‐PAGE indicated similarities between some of the phages but one canine phage was distinctly different, as was its morphology which was an isometric head with a short tail compared to oval heads and long tails which characterized others. IPS phages in the same serologic group had similar protein profiles but no correlation was observed with lytic groups. The use of protein profile and electron micrographs allowed classification of the phages into serogroups.It is concluded that the newly isolated canine phages could be very useful in typing Nigerian canine strains ofS. au
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