AbstractA 20·5 kb DNA fragment from the left arm of chromosome XI ofSaccharomyces cerevisiaehas been sequenced and analysed. Thirteen open reading frames (ORFs) for proteins longer than 100 amino acids were discovered. Among them, two are the known genesMRP49andTPK3; two others encode proteins which show strong similarity with a yeast putative protein kinase and a yeast choline transport protein; one other shows weaker similarity with a yeast Ca2+/calmodulin‐dependent protein kinase. Moreover, two putative proteins encoded by ORFs located in the sequenced fragment are closely similar to non‐yeast proteins: theCaenorhabditis eleganselongation factor 2 and a glutamic acid‐rich protein ofPlasmodium falciparum. The sequence has been deposited in the EMBL data library under Accession Number