Modified Gomori Trichrome Stain for Frozen Skeletal Muscle and Paraffin Embedded Sections
作者:
GarveyW.,
BigelowF.,
FathiA.,
JimenezC.,
CarpenterB.,
期刊:
Journal of Histotechnology
(Taylor Available online 1996)
卷期:
Volume 19,
issue 4
页码: 329-333
ISSN:0147-8885
年代: 1996
DOI:10.1179/his.1996.19.4.329
出版商: Taylor&Francis
关键词: cytoplasmic inclusion bodies;elastic fibers;frozen skeletal muscle;modified Gomori;nemaline rods;nuclei
数据来源: Taylor
摘要:
AbstractA modified Gomori technique for the assessment of frozen skeletal muscle and all paraffin embedded sections is presented. The modified Gomori solution consists of 0.015% fast green FCF, 0.12% chromotrope 2R, 0.65% phosphotungstic acid and 1.0% acetic acid in 100 ml distilled water. Frozen skeletal muscle staining of nuclei is done with celestine blue followed by hematoxylin; paraffin sections are stained with a modified Verhoeff hematoxylin. This method allows demonstration of nemaline rods, cytoplasmic inclusion bodies and other abnormalities in frozen skeletal muscle and takes about 12 minutes to perform. In paraffin sections the staining results are similar to the Masson trichrome method with less manipulation, cleaner background and staining time is about 17 minutes. (The J Histotechnol19:329–333, 1996)
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