Glycol Methacrylate Embedding for Light Microscopy: Basic Principles and Trouble-Shooting
作者:
GerritsPeter O.,
HorobinRichard W.,
期刊:
Journal of Histotechnology
(Taylor Available online 1996)
卷期:
Volume 19,
issue 4
页码: 297-311
ISSN:0147-8885
年代: 1996
DOI:10.1179/his.1996.19.4.297
出版商: Taylor&Francis
关键词: acrylic resins;embedding;glycol methacrylate;immunohistochemistry;tissue processing
数据来源: Taylor
摘要:
AbstractAcrylic resin mixtures are now widely used as embedding media for the preparation of tissue sections. Most of these mixtures are based on 2-hydroxyethyl methacrylate (glycol methacrylate, GMA). Resin embedding preserves tissue components far better than paraffin, celloidin or frozen sections. The present review describes the basic principles and trouble shooting, in particular: the chemical and physical properties of GMA, and components used for GMA mixtures; fixation of tissues for resin embedding; methods for dehydration; microtomy; stretching on water and mounting in relation to the final dimensions of GMA sections; staining of GMA 3embedded tissue sections; and the use of GMA resins in immunohistochemistry. In addition, standard, step by step procedures for embedding tissues in GMA is included. (The J Histotechnol19:297–311, 1996)
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