Selection and micropropagation of high artemisinin producing clones ofArtemisia annuaL.
作者:
H. M. Elhag,
M. M. El‐Domiaty,
F. S. El‐Feraly,
J. S. Mossa,
M. M. El‐Olemy,
期刊:
Phytotherapy Research
(WILEY Available online 1992)
卷期:
Volume 6,
issue 1
页码: 20-24
ISSN:0951-418X
年代: 1992
DOI:10.1002/ptr.2650060106
出版商: John Wiley&Sons, Ltd.
关键词: Artemisia annuaL;micropropagation;artemisinin;antimalarial drugs;sesquiterpene lactones;HPLC analysis;tissue cultures
数据来源: WILEY
摘要:
AbstractAnin vitromicropropagation method has been used to cloneArtemisia annuaL. plants from axenic seedlings or field grown plants selected on the basis of morphological characteristics during the growing season. Clones were maintained in culture by continuous transfer every 4–6 weeks. The performance of the various clones in the field as well as their contents of artemisinin and related sesquiterpenes were compared with selected field grown plants. An internally standardized reverse‐phase HPLC method was developed for this evaluation. Artemisinic acid (III) was by far the major sesquiterpene for all clones tested, followed by arteannuin B (II) and then artemisinin (I). However, there was a high degree of variability between various clones for all sesquiterpenes tested. The high artemisinin producing clones are characterized as tall robust plants with long internodes, open branching, dense leaves and thick stems. Results from unorganized callus cultures initiated from the leaves of the corresponding clones did not indicate the formation of artemisinin or related sesquiterpenes in these cultu
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