A rapid method forin situhybridization for viral DNA in brain biopsies from patients with AIDS
作者:
Sidney Houff,
David Katz,
Conrad Kufta,
Eugene Major,
期刊:
AIDS
(OVID Available online 1989)
卷期:
Volume 3,
issue 12
页码: 843-846
ISSN:0269-9370
年代: 1989
出版商: OVID
关键词: in situhybridization;viral infection of central nervous system;brain biopsy
数据来源: OVID
摘要:
Brain biopsy is often necessary in the diagnosis of neurological complications found in AIDS patients. We describe here a rapid method of tissue preparation andin situDNA hybridization for detecting JC virus DNA in frozen brain biopsy sections which allows the diagnosis of progressive multifocal leukoencephalopathy to be established on the day of surgery. Once the diagnosis is established, therapeutic and management decisions can be made more easily. The commercial availability of biotinylated probes for several of the DNA viruses most frequently encountered in brain infections of AIDS patients will provide wide application of these techniques to patient management.
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