Use of Dark-Field and Fluorescence Microscopy to Examine Extracellular Hyaline Accumulation in the Mouse
作者:
Robert J. Wordinger,
期刊:
Cells Tissues Organs
(Karger Available online 1988)
卷期:
Volume 132,
issue 4
页码: 327-330
ISSN:1422-6405
年代: 1988
DOI:10.1159/000146597
出版商: S. Karger AG
关键词: Hyaline;Dark-field microscopy;Fluorescence microscopy;Diethylstilbestrol
数据来源: Karger
摘要:
Small foci of diethylstilbestrol-induced extracellular hyaline material were visualized within the mouse uterus utilizing dark-field and fluorescence microscopy following conventional hematoxylin and eosin staining. Hyaline was observed throughout the connective tissue stroma as well as between smooth muscle cells of the myometrium. The use of these techniques permits the rapid examination of small aggregates of hyaline material which are not visible with conventional hematoxylin and eosin staining and eliminates the need for time-consuming and complicated histochemical techniques. The procedures are simple and may also facilitate the quantitative (morphometric) analysis of this material.
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