HISTOCOMPATIBILITY MATCHING VI. MINIATURIZATION OF THE MIXED LEUKOCYTE CULTURE TESTA PRELIMINARY REPORT
作者:
R. HARTZMAN,
M. SEGALL,
M. BACH,
F. BACH,
期刊:
Transplantation
(OVID Available online 1971)
卷期:
Volume 11,
issue 3
页码: 268-273
ISSN:0041-1337
年代: 1971
出版商: OVID
数据来源: OVID
摘要:
A miniaturized method for the mixed leukocyte culture (MLC) test is described which results in substantial savings in cells, medium, and time. The test is done in microtiter plates using 0.2-ml culture volumes. Concentrations of responding and stimulating cells are varied, depending on the experiment. Significant discrimination between isogeneic and allogeneic mixtures is possible when cultures are labeled for a 16–20-hr period starting at 48 hr. The method measures MLC identity, as previously defined, and quantitates in a manner significantly correlated with the macro method.
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