The “exposed plate” method was used to trap fungal spores from the atmosphere of Wadi Qena over a period of one year (January-December 1985). 83 species and 2 varieties representing 31 genera were collected. The most frequently isolated genera on mesophilic and osmophilic media includedAlternaria, Aspergillus, Cladosporium, Cumularia, Drechslera, Epicoccum, Penicillium, StemphyliumandUlocladium; their counts fluctuated irregularly.Alternaria altérnala, Aspergillus nidulans, A. niger, A. sydowi, A. terreus, Cladosporium cladosporioides, C. herbarum, Cumularia lunata, Drechslera spicifera, Epicoccum purpurascens, Penicillium chrysogenum, P. citrinum, Stemphylium botryosumandUlocladium botrytiswere the most prevalent species. Also,Eurotium(represented byE. amstelodami, E. chevalieriandE. rubrum) was only encountered frequently on osmophilic medium. On halophilic mediumAspergillus sydowiwas common followed byAspergillus nidulans, A. niger, A. terreusandCladosporium herbarum. Fungi isolated have been tested for osmophilic and halophilic ability and they showed different rates of growth on sucrose- and sodium chloride-Czapek's medium of various osmotic potential. Members ofAspergillusandEurotiumwere considerably the most resistant and able to grow on sodium chloride agar medium of osmotic potential reached to -175.7 or -218.45 bars.