Resonance Raman Studies of Copper Pheophytinate
作者:
JamesS. Keller,
JohnJ. McMahon,
Raymond Chang,
RobertL. Carter,
期刊:
Spectroscopy Letters
(Taylor Available online 1984)
卷期:
Volume 17,
issue 2
页码: 145-149
ISSN:0038-7010
年代: 1984
DOI:10.1080/00387018408068052
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
In recent years there has been considerable interest in the spectroscopic properties of chlorophyll molecules. Among the techniques employed is resonance Raman spectroscopy which can provide vibrational spectra with high selectivity, from chlorophyllapresent in intact chloroplasts or cells.1However, detecting resonance Raman scattering from chlorophyllais hindered when excitation in the red region is used, owing to the strong fluorescence of the molecule near 670 nm. Copper pheophytinate, on the other hand, is largely non-fluorescent and thus lends itself to resonance Raman study in the red region. (The mechanism for fluorescence quenching in copper pheophytinate is believed to involve charge transfer from the porpnyrin ring to the Cu2+ion upon excitation, although direct experimental evidence is still lacking.) we felt that it would be of interest to carry out a resonance Raman study to copper pheophytinate in the red region and compare the results with what is already known about the resonance raman sperctrum of chlorophy 11a. An advantage of exciting in the red region is that athe QXand Qybamus in the red region are better resolved than the BXand Bybands in the blue region,2thus making it easier to observe different vibronic coupling in the red.
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