首页   按字顺浏览 期刊浏览 卷期浏览 Morphology of adultPhyllodistomurn umblae(Fabricius) (Platyhelminthes, Gorgoderidae): t...
Morphology of adultPhyllodistomurn umblae(Fabricius) (Platyhelminthes, Gorgoderidae): the effect of preparation, killing and fixation procedures

 

作者: TOR A. BAKKE,  

 

期刊: Zoologica Scripta  (WILEY Available online 1988)
卷期: Volume 17, issue 1  

页码: 1-13

 

ISSN:0300-3256

 

年代: 1988

 

DOI:10.1111/j.1463-6409.1988.tb00082.x

 

出版商: Blackwell Publishing Ltd

 

数据来源: WILEY

 

摘要:

Twenty two different handling techniques for determining the morphology ofPhyllodislormum umblaerecovered from the Arctic char,Salvelinus alpinus, in Norway are examined. In some procedurcs both hot and cold fixation were used. Examples of different methods are illustrated by photographs. In addition, the natural morphological variability ofP. umblaeis demonstrated based on specimens from different hosts and localities in Norway and Sweden. The treatment procedures fall within four main groups: (1) heat fixation, (2) cold fixation, (3) anaesthetization (physical, chemical) and (4) fixation under pressure. It is concluded that only methods which give stretching (and swelling) are passable if acceptable specimen, for measurement and descriptions are to be obtained. This is due to the obscurity of important characters in fluke bodies which are often curled or contracted whcn ‘naturally displayed’ on slides. The acceptable methods for gorgoderid taxonomy based on gross morphology in total mounts are: (1) heat fixation (based on formalin, alcohol and glacial acetic acid), (2) cold fixation (based on glacial acetic acid or Berland's fluid) and (3) anaesthetization (based on heating or freezing in saline or chemical relaxation). The recommended methods are: (1) 10% hot buffered formalin‐saline. if heating facilities are available, or, if not, (2) Berland's

 

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