AbstractA collection of infectious pancreatic necrosis virus (IPNV) isolated in five provinces of eastern Canada was analysed by an antigen‐coated immunosorbent assay and by neutralization tests using selected monoclonal and polyclonal antibodies. Relevant antigenic sites of the two major capsid proteins, VP2 and VP3, were simultaneously compared. The A1 serotype was predominant and no significant variations of VP2 epitopes were observed. However, two subtypes could be distinguished on the basis of one or two epitopes on VP3. Other serotypes such as A6, A7 or A8 have been detected in piscicultures of New Brunswick and Nova Scotia. The antigenic characterization of IPNV strains appears of interest for epidemiological studie