Simultaneous Determination of Flavonoids, Phenolic Acids, and Coumarins in Seven Medicinal Species by HPLC/DIODE-Array Detector
作者:
P.B. Andrade,
R.M. Seabra,
P. Valentão,
F. Areias,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1998)
卷期:
Volume 21,
issue 18
页码: 2813-2820
ISSN:1082-6076
年代: 1998
DOI:10.1080/10826079808003444
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A simple and accurate reversed phase HPLC procedure is proposed for the determination of 19 phenolic compounds (flavonoids, phenolic acids, and coumarins) in seven medicinal species. The sample preparation involved extraction, alkaline and acid hydrolysis, and purification through a Bond-Elut C18 column. The chromatographic separation was achieved using a reversed phase column Spherisorb ODS2 (5μm; 25.0 × 0.46 cm). Gradient elution was carried out using water-formic acid (19:1) (A) and methanol (B). A diode-array detector monitored the effluent and chromatograms were recorded at 280, 320, and 350 nm. The optimised methodology seems to be useful for the phytochemical analysis of plant extracts. A close correlation between the phenolic compound patterns and the botanical origin of plants was found.
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