Sex‐, tissue‐, and stage‐specific expression of a vitelline membrane protein gene from region 32 of the second chromosome ofDrosophila melanogaster
作者:
S. Gigliotti,
F. Graziani,
L. De Ponti,
F. Rafti,
A. Manzi,
G. Lavorgna,
G. Gargiulo,
C. Malva,
期刊:
Developmental Genetics
(WILEY Available online 1989)
卷期:
Volume 10,
issue 1
页码: 33-41
ISSN:0192-253X
年代: 1989
DOI:10.1002/dvg.1020100106
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Oogenesis;Eggshell;Gene family
数据来源: WILEY
摘要:
AbstractThis study isolated cDNA clones from egg‐chamber and adult female Drosophila cDNA libraries using as probe a DNA fragment from a 200‐kb “chromosome walk” in region 32E of the second chromosome of D. melanogaster. The present authors believe that these clones correspond to a new vitelline membrane protein (VMP) gene because (1) cDNA clones in Northern blots identify a transcript expressed in a tissue‐ and stage‐specific manner: stage 10 egg‐chambers; (2) the sequence of cDNAs and of the genomic subclone shows homology with the other VMP genes that have been identified to date; (3) the amino acid composition of the translational product has the high content of proline and alanine characteristic of VMPs. Two aspects emerging from this study are worth stressing: (1) the presence of a hydrophobic domain that is highly conserved in all the VMP genes; and (2) the particularly narrow period of expression of the isolated gene, which could be related to the mechanism of vitelline memb
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