Analysis of the genome of molluscum contagiosum virus by restriction endonuclease analysis and molecular cloning
作者:
G. Darai,
H. Reisner,
J. Scholz,
P. Schnitzler,
H. Lorbacher de Ruiz,
期刊:
Journal of Medical Virology
(WILEY Available online 1986)
卷期:
Volume 18,
issue 1
页码: 29-39
ISSN:0146-6615
年代: 1986
DOI:10.1002/jmv.1890180105
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: poxvirus DNA;recombinant plasmid;typing of restriction endonucleases
数据来源: WILEY
摘要:
AbstractVirions of molluscum contagiosum virus (MCV), a member of the poxviridae, were isolated directly from lesions of individual patients and characterized by restriction enzyme analysis. The comparative analysis of the cleavage patterns and Southern blot hybridization of 14 independently isolated virus samples revealed that MCV isolates can be classified into two different types. The majority of MCV isolated from clinically typical skin lesions (13 of 14) showed similar DNA cleavage patterns and were termed MCV type 1, whereas one isolate derived from a vaginal lesion showed a completely different DNA cleavage pattern and therefore was termed MCV type 2. For detailed investigation of the viral genome, a defined gene library of MCV DNA sequences was established. The Bam HI DNA fragments of the viral genome of MCV type 1 prototype isolate 1/80 was inserted into the bacterial plasmid vector pAT153. With the exception of terminal fragments (fragments A and B) of the viral genome, all other DNA fragments were cloned. All cloned Bam HI DNA fragments were individually identified by digestion of the recombinant plasmid DNA with different restriction enzymes and screened by hybridization of plasmid DNA to viral DNA.
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