Clinical Application of Flow Cytometry for DNA Analysis of Solid Tumors
作者:
Kohsuke Sasaki,
Tomoyuki Murakami,
期刊:
Pathology International
(WILEY Available online 1992)
卷期:
Volume 42,
issue 1
页码: 1-14
ISSN:1320-5463
年代: 1992
DOI:10.1111/j.1440-1827.1992.tb01105.x
出版商: Blackwell Publishing Ltd
关键词: Flow cytometry;Cancer;DNA;Ploidy;Cell kinetics
数据来源: WILEY
摘要:
Recent developments of flow cytometry (FCM) technology which make multiple correlative biological measurements on normal and neoplastic cells is affecting areas of diagnostic pathology as well as research fields, and a general understanding of FCM techniques is essential for pathologists. Today, FCM DNA measurement of tumors also becomes routine in the clinical and/or pathological laboratory for aid in cancer diagnosis and cancer treatment. It can also contribute to diagnosis of tumors as a supplemental method to conventional histopathology, and DNA ploidy and the percentages of S‐phase fraction are considered as complementary prognostic parameters independent of the stage of disease. This article reviews clinical applications of flow cytometry focusing on the DNA measurements of solid tumors, and related practical issues, such as the methodology for nuclear DNA measurement, interpretation of DNA histograms and the relationship of DNA ploidy and S phase fraction to clinical and pathological features of human solid tumor
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