Interaction of Adenosine and Adenylnucleotides with the Human Platelet Membrane. Further Characterization of the ADP Binding Sites
作者:
Brigitte Bauvois,
Chantal Legrand,
Jacques P. Caen,
期刊:
Pathophysiology of Haemostasis and Thrombosis
(Karger Available online 1980)
卷期:
Volume 9,
issue 2
页码: 92-104
ISSN:1424-8832
年代: 1980
DOI:10.1159/000214346
出版商: S. Karger AG
关键词: Adenosine;Adenylnucleotides;ADP binding sites;Platelet membrane
数据来源: Karger
摘要:
The binding of adenosine and adenylnucleotides to the human platelet membrane has been measured and the subsequent effect on the binding of 14C-ADP has been determined. The following conclusions have been drawn: (1) Two reactions with different velocities (t 1/2 = 15–30 sec and t 1/2 = 4–6 min) seem to be involved in the binding of ADP to the platelet membrane. (2) AMP binds to the platelet membrane and partially inhibits binding of ADP in a competitive manner. These two findings suggest that ADP binding sites on the platelet membrane may be of more than one type. (3) At concentrations up to 10-5 M, ATP does not itself bind to the platelet membrane but is rapidly transformed into ADP which, in turn, binds to the membrane and inhibits the binding of 14C-ADP. (4) Adenosine does not significantly interfere with the binding of 14C-ADP and has not been shown to bind to the platelet membr
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