Circadian rhythmicity of tyrosine aminotransferase activity in larval and prepupal fat body ofDrosophila melanogaster
作者:
W. Volknandt,
R. Hardeland,
期刊:
Journal of Interdisciplinary Cycle Research
(Taylor Available online 1978)
卷期:
Volume 9,
issue 4
页码: 283-291
ISSN:0022-1945
年代: 1978
DOI:10.1080/09291017809359644
出版商: Taylor & Francis Group
关键词: Circadian rhythms;Drosophila melanogaster;fat body;larvae;prepupae;tyrosine aminotransferase
数据来源: Taylor
摘要:
Drosophila melanogasterwas reared under LD 12:12. Fat body was isolated in form of cells and cell groups from dissected larvae or prepupae by treatment with collagenase and hyaluronidase and subsequent centrifugation. Tyrosine aminotransferase of the fat body showed an extremely sharp pH dependence, with two narrow, well separable maxima at pH 5.6 and 6.0, possibly caused by different isoenzymes. Enzyme activity at pH 5.6 exhibited biphasic circadian rhythms in both wild‐type 3rd instar larvae and early prepupae. At pH 6.0, the rhythmicity was much weaker, perhaps even absent. In larvae from an ebony strain, at either pH the basal tyrosine aminotransferase activity was enhanced, and the rhythmicity was altered.
点击下载:
PDF (487KB)
返 回