Purification of arginine‐sensitive hemagglutinin fromFusobacterium nucleatumand its role in coaggregation
作者:
T. Takemoto,
M. Ozaki,
M. Shirakawa,
T. Hino,
H. Okamoto,
期刊:
Journal of Periodontal Research
(WILEY Available online 1993)
卷期:
Volume 28,
issue 1
页码: 21-28
ISSN:0022-3484
年代: 1993
DOI:10.1111/j.1600-0765.1993.tb01046.x
出版商: Blackwell Publishing Ltd
关键词: hemagglutinin;arginine‐sensitivity;coaggregation;Fusobacterium nucleatum
数据来源: WILEY
摘要:
Hemagglutinin ofFusobacterium nucleatumwas extracted from Triton X‐100‐pronase P‐treated cell envelopes, and was purified by affinity chromatography on L‐arginine agarose. The hemagglutinin was inactivated by heating at 70°C for 1 min. The activity was inhibited by L‐arginine but was not affected by any sugars or by EDTA. The hemagglutinin aggreggated 14 out of 17 strains of oral streptococci tested, and the bacterial aggregating activity was also inhibited by L‐arginine. The results indicate the dominant role of this hemagglutinin in the adherence of this bacterium both to host cells and to ot
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