Ultrastructural Characterization of Isolated Human Head and Neck Squamous Carcinoma Cells. Assessment of Isopycnic Centrifugation
作者:
Vernon KingMurray,
ParsonsDonald F.,
期刊:
Cancer Investigation
(Taylor Available online 1988)
卷期:
Volume 6,
issue 1
页码: 55-65
ISSN:0735-7907
年代: 1988
DOI:10.3109/07357908809077029
出版商: Taylor&Francis
数据来源: Taylor
摘要:
AbstractIn an experiment to evaluate the merit of isopycnic centrifugation as a method of separating cell types in human head and neck squamous cell carcinomas, cells have been isolated from four specimens of these tumors and subjected to isopycnic centrifugation in continuous Percoll gradients. Cell types were identified by electron microscopy. The R- (ribosome-rich), T- (tonofilament-rich), and RT- (intermediate) cell types yielded broad bands overlapping extensively with one another, and partially with the bands of leukocytes. The pattern differed for each tumor studied, so that universal density levels separating given cell types could not be found. Isopycnic centrifugation proves less suitable in analyzing cells dispersed from solid tumors than for cells in suspended culture, blood, effusions, etc., probably because of heterogeneous growth conditions of cells in solid tumors.
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