PCR probes for chromosome in situ hybridization of large-insert bacterial recombinants
作者:
P.M. Kroisel,
P.A. loannou,
P.J. de Jong,
期刊:
Cytogenetic and Genome Research
(Karger Available online 1994)
卷期:
Volume 65,
issue 1-2
页码: 97-100
ISSN:1424-8581
年代: 1994
DOI:10.1159/000133609
出版商: S. Karger AG
数据来源: Karger
摘要:
We have developed a procedure for efficient in situ hybridization of bacterial recombinants created with various types of large-insert cloning vectors. Minimal quantities of crude DNA are amplified and labeled during the degenerate-oligonucleotide-primed polymerase chain reaction. The resulting probes generate high-intensity fluorescent hybridization signals on metaphase chromosomes and on interphase nuclei.
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