Tobacco in Malawi often suffers from outbreaks of anthracnose caused byColletotrichum tabacum(Boning); particularly at the seedling stage. In order to identify suitable sources of resistance for use in a breeding programme three varieties ofNicotiana tabacumand eight otherNicotianaspecies were tested in the greenhouse trials, three varieties ofN. tabacumand five otherNicotianaspecies were tested in seedbed trials. The most promising sources appeared to beN. sanderaeorN. debneyi, and possiblyN. nudicaulisorN. forgetiana.