Cholesterol-Rich Microdomains in Rat and Porcine Thyroid Membranes Involved in TSH-Induced Endocytotic Processes
作者:
H. Barrière,
B. Chailley,
M. Chambard,
J.P. Selzner,
J. Mauchamp,
J. Gabrion,
期刊:
Cells Tissues Organs
(Karger Available online 1988)
卷期:
Volume 132,
issue 3
页码: 205-215
ISSN:1422-6405
年代: 1988
DOI:10.1159/000146575
出版商: S. Karger AG
关键词: Cholesterol;Filipin;Epithelial thyroid cells;Endocytosis;Pseudopod membrane;Freeze-fracture
数据来源: Karger
摘要:
Filipin, a polyene antibiotic, was used to detect cholesterol in thyroid membranes in vivo and in culture duringTSH stimulation. We found that apical and basolateral plasma membranes were heterogeneously modified by filipin which induced abundant lesions in apical membranes, whereas Golgi apparatus, endoplasmic reticulum, nuclear membranes were unmodified. Small apical vesicles and colloid droplets were generally highly enriched in these complexes, suggesting a high cholesterol concentration in their membranes. Pseudopod membranes, known to be highly specialized domains in the apical plasma membrane, appeared enriched in cholesterol. Consequently, we suggest that an increased cholesterol content may be involved in the stabilization of thyroid membranes during endocytotic processes.
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