Comparison of two separation techniques for the determination of blood mononuclear cell magnesium content.
作者:
HosseiniJ M,
JohnsonE,
ElinR J,
期刊:
Journal of the American College of Nutrition
(Taylor Available online 1983)
卷期:
Volume 2,
issue 4
页码: 361-368
ISSN:0731-5724
年代: 1983
DOI:10.1080/07315724.1983.10719933
出版商: Routledge
数据来源: Taylor
摘要:
The determination of blood mononuclear cell magnesium content may be a predictor of total body magnesium status. Separation techniques employing Ficoll-Hypaque have been used for this assay; however, Hypaque contains a significant concentration of iodine that may be toxic to the cell membrane and affect the results. We developed a different separation technique using arabinogalactan (Stractan) at concentrations of 13.0 and 17.7%. The blood from 16 normal volunteers was divided and assayed for mononuclear cell magnesium content using both methods. The results (mean +/−standard deviation) with the Ficoll-Hypaque method were 83.6 +/−30.8 fg/cell, and with the arabinogalactan method they were 78.4 +/−25.5 fg/cell. A paired sample t test comparing the results between the two methods gave a t value of 1.05 (P greater than .3). The purest population of blood mononuclear cells was obtained with the Ficoll-Hypaque method. Thus, these two separation materials do not have a significant effect on the blood mononuclear cell magnesium content, and the better separation of cells was obtained with Ficoll-Hypaque.
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